海藻糖生物特性及其在ELISA技术研发中的应用

›› 2012, Vol. 39 ›› Issue (10): 69-72.

海藻糖生物特性及其在ELISA技术研发中的应用

王磊, 崔东安, 张景艳, 李建喜

中国农业科学院兰州畜牧与兽药研究所,农业部新兽药工程重点实验室, 甘肃省中兽药工程技术研究中心,甘肃兰州 730050

收稿日期:2012-02-29 出版日期:2012-10-20 发布日期:2012-10-19
通讯作者: 李建喜(1971-),男,甘肃人,博士,副研究员,研究方向:兽医药理与毒理学。E-mail:lzjianxil@163.com E-mail:lzjianxil@163.com
作者简介:王磊(1985-),女,河南人,硕士生,研究方向:兽医药理与毒理学。
基金资助:
甘肃省科技支撑计划项目(1011NKCA048);中央级公益性科研院所基本科研业务费专项资金项目(BRF09030)。

Biological Properties of Trehalose and its Application in ELISA

WANG Lei, CUI Dong-an, ZHANG Jing-yan, LI Jian-xi

Engineering & Technology Research Center of Traditional Chinese Veterinary Medicine of Gansu Province, Key Laboratory of Veterinary Pharmaceutical Development, Ministry of Agriculture, Lanzhou Institute of Husbandry and Pharmaceutics of CAAS, Lanzhou 730050,China

Received:2012-02-29 Online:2012-10-20 Published:2012-10-19

摘要/Abstract

摘要： 海藻糖属非还原性双糖,在生物体内作为一种贮藏性糖类,可提供能量来源,同时它也是一种重要的应激代谢产物,对应激状态具有高度抗性。外源性海藻糖对细胞、抗体等生物活性物质同样具有非特异性生物保护作用。文章综述了海藻糖的能量储备、抗应激、细胞稳定剂等生物学特性,以及对细胞、抗体和ELISA中包板抗原或抗体的保护作用,并对其在ELISA技术改善中的应用前景做了推测与展望。

关键词: 海藻糖; 生物学特性; ELISA

Abstract: Trehalose is a non-reducing disaccharide whose function in some organisms is as a source of energy or a protectant against the stress. The exogenous trehalose is contributed to protecting the bioactivitie of some substances like cells and antibodies in vitro. This review summarizes the biological properties of trehalose, such as energy source, stress resistance, cell stabilizer and helping to stabilize antigens or antibodies in ELISA. The trehalose is also helpful for improving ELISA detection.

Key words: trehalose; biological properties; ELISA

中图分类号:

Q946.3

王磊, 崔东安, 张景艳, 李建喜. 海藻糖生物特性及其在ELISA技术研发中的应用[J]. , 2012, 39(10): 69-72.

WANG Lei, CUI Dong-an, ZHANG Jing-yan, LI Jian-xi. Biological Properties of Trehalose and its Application in ELISA[J]. , 2012, 39(10): 69-72.

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