基于等位基因特异性PCR原理建立鸡白痢沙门氏菌PCR方法

›› 2011, Vol. 38 ›› Issue (5): 93-96.

基于等位基因特异性PCR原理建立鸡白痢沙门氏菌PCR方法

涂玉蓉^1,2, 陈建红², 任涛¹, 张济培², 司兴奎², 牛森^1,2

1. 佛山科学技术学院动物医学系,广东佛山 528231;2. 华南农业大学兽医学院,广东广州 510642

收稿日期:2010-11-09 修回日期:1900-01-01 出版日期:2011-05-20 发布日期:2011-05-20
通讯作者: 陈建红

Development PCR Assay for Detecting Salmonella pullorum Based on Allele-specific PCR

TU Yu-rong^1,2, CHEN Jian-hong², REN Tao¹, ZHANG Ji-pei², SI Xing-kui², NIU Sen^1,2

1. College of Life Science,Foshan University,Foshan 528231,China;2. College of Veterinary Medicine,South China Agricultural University,Guangzhou 510642,China

Received:2010-11-09 Revised:1900-01-01 Online:2011-05-20 Published:2011-05-20

摘要/Abstract

摘要： 根据鸡白痢沙门氏菌与鸡伤寒沙门氏菌的rfbS基因在第237和598位碱基的不同,设计和合成等位基因特异性PCR引物,建立快速检测鸡白痢沙门氏菌的PCR方法,并应用该法对鸡白痢沙门氏菌临床分离样品进行了PCR鉴定。结果显示,该PCR方法能特异性地鉴定鸡白痢沙门氏菌,检测灵敏度达18 pg/μL DNA,4.7×10⁴ CFU/mL菌液,表明建立的等位基因特异性PCR方法能准确而快速地鉴定鸡白痢沙门氏菌。

关键词: 等位基因特异PCR; 鸡白痢沙门氏菌; rfbS基因

Abstract: Based on the nuclei polymorphism of rfbS gene sequence of Salmonella pullorum at site 237 and 598 compared with S.gallinarum,a pair of allele-specific primers were designed and synthesized,and an allele-specific PCR method for detecting S.pullorum was developed consistent with result of the serological and biochemical method and the sensitivity of the PCR assay was 18 pg DNA and 4.7×10⁴ CFU/mL cultural liquid.The results showed that the developed allele-specific PCR was a very rapid,sensitive and specific molecular tool for the detection and identification of S.pullorum isolates.

Key words: allele-specific PCR; Salmonella pullorum; rfbS gene

中图分类号:

S858.31

涂玉蓉;陈建红;任涛;张济培;司兴奎;牛森;. 基于等位基因特异性PCR原理建立鸡白痢沙门氏菌PCR方法[J]. , 2011, 38(5): 93-96.

TU Yu-rong;CHEN Jian-hong;REN Tao;ZHANG Ji-pei;SI Xing-kui;NIU Sen;. Development PCR Assay for Detecting Salmonella pullorum Based on Allele-specific PCR[J]. , 2011, 38(5): 93-96.

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