›› 2011, Vol. 38 ›› Issue (2): 149-151.

• 疾病防治 • 上一篇    下一篇

致病性水貂肠炎病毒MEV-ZJ1株的分离与鉴定

谭斌1,陈微晶2,武华1,2   

  1. (1.中国农业科学院特产研究所人兽共患病研究室,吉林吉林 132109;2.北京必威安泰生物科技有限公司,北京 100176)
  • 收稿日期:1900-01-01 修回日期:1900-01-01 出版日期:2011-02-20 发布日期:2011-02-20
  • 通讯作者: 武华

Isolation and Identification of Pathogenic Mink Enteritis Virus MEV-ZJ1 Strain

TAN Bin1, CHEN Wei-jing2, WU Hua1,2   

  1. (1.Division of Zoonosis, Institute of Special Economic Animal and Plant Sciences, Chinese Academy of Agricultural Sciences, Jilin 132109, China;2.Beijing Bigvet Biotech Co.Ltd., Beijing 100176, China)
  • Received:1900-01-01 Revised:1900-01-01 Online:2011-02-20 Published:2011-02-20
  • Contact: WU Hua

摘要: 自临床疑似水貂病毒性肠炎发病貂的粪便中分离出1株病毒,经病毒形态观察、理化特性检测、血清学、聚合酶链式反应(PCR)和动物试验鉴定表明,该分离毒为水貂肠炎病毒(mink enteritis virus,MEV),命名为MEV-ZJ1株。用分离毒接种水貂,试验动物均表现出典型水貂病毒性肠炎临床症状。研究表明,MEV-ZJ1分离株对水貂具有致病性,是1株强毒,为进一步开展该病毒流行病学、致病机理、疫苗免疫与诊断检测的研究奠定了基础。

关键词: 水貂肠炎病毒; 强毒; 分离与鉴定

Abstract: A virus strain was isolated from feces of a mink suspected MEV infection. It was demonstrated to be MEV by morphology, physical and chemical properties, serum test, PCR and animal test,and it was named MEV-ZJ1. Minks were artificially infected by MEV-ZJ1 developed obvious symptoms of enteritis post-inoculation.The MEV-ZJ1 strain was virulent for minks. The successful isolation of MEV-ZJ1 provided a basis for further search on epidemiology, pathogenesis, vaccination and diagnostic.

Key words: mink enteritis virus; virulent virus; isolation and identification

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