C6细胞HGPRT缺陷株诱变的试验研究

›› 2011, Vol. 38 ›› Issue (12): 50-51.

C6细胞HGPRT缺陷株诱变的试验研究

王新^1,2, 孙书芳³, 谭建华⁴, 赖小平²

1. 青岛农业大学动物科学技术学院,山东青岛 266109;2. 广州中医药大学中药学院,广东广州 510006;3. 山东省青岛市中心血站,山东青岛 266071;4. 解放军军事医学科学院军事兽医研究所,吉林长春 130062

收稿日期:2011-05-09 修回日期:1900-01-01 出版日期:2011-12-20 发布日期:2011-12-20
通讯作者: 谭建华, 赖小平

Study on Mutagenicity of C6 Cells HGPRT^- Lines

WANG Xin^1,2, SUN Shu-fang³, TAN Jian-hua⁴, LAI Xiao-ping²

1. College of Animal Science,Qingdao Agricultural University,Qingdao 266109,China;2. Traditional Chinese Medical Academy of Guangzhou University of Chinese Medicine,Guangzhou 510006,China;3. Qingdao Blood Center, Qingdal 266071,China;4. Institute of Military Veterinary Academy of Military Medical Science,PLA,Changchun 130062,China

Received:2011-05-09 Revised:1900-01-01 Online:2011-12-20 Published:2011-12-20

摘要/Abstract

摘要： 通过N-甲基-N'-硝基亚硝基胍、8-氮鸟嘌呤、6-巯基鸟嘌呤紫外线照射的联合作用,试图建立C6细胞的次黄嘌呤鸟嘌呤磷酸核糖基转移酶缺陷株,为建立大鼠垂体促性腺激素杂交瘤细胞株奠定基础,但最终未能获得预期的能够被HAT培养基筛选的HGPRT-缺陷株,提示C6细胞株的HGPRT基因非常稳定,诱变方法难以使其突变缺失。

关键词: MNNG; 8-AG; 6-TG; HGPRT; C6细胞

Abstract: This study tries to product rat gloma cells hypox anthine-guaninep hosphoribosyl transferase deficiency lines by combined effects of N–methyl-N＇–nitro-nitrosoguanidine,8-azaguanine,6-thioguanine and ultraviolet irradiation in order to establish rat pituitary gonadotropic hormone hybridoma cell lines in future,but wo are not able to gain the expectant glioma HGPRT^- cell lines which can be sieved by hypoxan thine-aminop terinthymidine selective medium.The experiment indicate that the HGPRT gene of C6 cells is too stabile to be induced deficient on this experiment conditions.

Key words: MNNG; 8-AG; 6-TG; HGPRT; C6 cells

中图分类号:

王新;孙书芳;谭建华;赖小平. C6细胞HGPRT缺陷株诱变的试验研究[J]. , 2011, 38(12): 50-51.

WANG Xin;SUN Shu-fang;TAN Jian-hua;LAI Xiao-ping. Study on Mutagenicity of C6 Cells HGPRT^- Lines[J]. , 2011, 38(12): 50-51.