›› 2011, Vol. 38 ›› Issue (10): 99-105.

• 遗传繁育 • 上一篇    下一篇

内蒙古白绒山羊附睾精子冷冻效果的研究

柴局1, 张燕军1, 张家新1, 高日明2, 孟克格日乐1, 王惠梅3, 张文广1, 李金泉1   

  1. 1. 内蒙古农业大学动物科学学院,内蒙古呼和浩特 010018;2. 内蒙古金宇保灵生物有限公司,内蒙古呼和浩特 010030;3. 内蒙古包头市石拐区农牧水利局,内蒙古包头 014070
  • 收稿日期:2011-03-18 修回日期:1900-01-01 出版日期:2011-10-20 发布日期:2011-10-20
  • 通讯作者: 李金泉

Studies on Effects of Cryopreserve Epididymal Spermatozoa Retrieved after Death on Cashmere Goat of Inner Mongolia

CHAI Ju1, ZHANG Yan-jun1, ZHANG Jia-xin1, GAO Ri-ming2, MENG Kegerile1, WANG Hui-mei3, ZHANG Wen-guang1, LI Jin-quan1   

  1. 1. College of Animal Science,Inner Mongolia Agricultural University,Hohhot 010018,China;2. Jinyu Paul Ling Biological Pharmaceutical Company Limited, Hohhot 010030, China;3. Agriculture and Animal Husbandry Bureau of Water Resources in Shiguai, Baotou 014070, China
  • Received:2011-03-18 Revised:1900-01-01 Online:2011-10-20 Published:2011-10-20

摘要: 本试验把不同温度下的样品分成3个处理组,通过检测精子与繁殖力有关的重要指标研究3个处理组的冷冻效果,结果显示, 在3个不同的环境下收集附睾,室温下(19 ℃)收集附睾精子(处理1组)的冷冻效果最好,解冻后活率为(55.0±3.4)%;4 ℃下收集的精子(处理2组)冷冻解冻后的活率为(35.0±2.5)%;37 ℃下收集的精子(处理3组)的冷冻效果较差,解冻后活率为(23.0±2.5)%。通过对处理组中冷冻和解冻后两个阶段的精子指标的测定,研究了精子活率与其他指标的关系,得出白绒山羊附睾精子能够成功的保存。

关键词: 内蒙古白绒山羊; 附睾精子; 精子收集; 冷冻保存

Abstract: In this study, experiment results showed that after postmortem cooling the environment in three different collection of epididymal and testicular frozen dead animals 22 hours later,at room temperature (19 ℃) collection of sperm (treatment 1) was the best effect, 55.0%± 3.4%motile, frozen-thawed sperm. At 4 ℃ to collect sperm motility (treatment 2) was 35.0%±2.5%, collected at 37 ℃ (treatment 3) sperm motility, was 23.0%±2.5%. Through two stages of treatment group indicators of sperm the determination of the sperm motility and sperm of other indicators of the relationship between the cashmere goats obtained sperm can successfully saved.

Key words: Inner Mongolia Cashmere goat; epididymal sperm; semen recovery; cryopreservation

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