表面等离子共振生物传感器检测培氟沙星的方法研究

›› 2010, Vol. 37 ›› Issue (8): 233-235.

表面等离子共振生物传感器检测培氟沙星的方法研究

郑晓华，魏星华，沈明浩

（吉林农业大学食品科学与工程学院，长春，130118）

收稿日期:1900-01-01 修回日期:1900-01-01 出版日期:2010-08-20 发布日期:2010-08-20
通讯作者: 沈明浩

The Methods of Detecting Pefloxacin by Surface Plasmon Resonance Biosensor

ZHENG Xiao-hua，WEI Xing-hua，SHEN Ming-hao

(College of Food Science and Engineering, Jilin Agricultural University, Changchun 130118,China)

Received:1900-01-01 Revised:1900-01-01 Online:2010-08-20 Published:2010-08-20
Contact: SHEN Ming-hao

摘要/Abstract

摘要： 研究表面等离子共振生物传感器检测缓冲液中培氟沙星的方法，为样品的检测提供依据。采用抑制法，将培氟沙星与卵清蛋白的偶联物固定在芯片上，将抗体与不同浓度的培氟沙星混合后通入芯片表面，优化反应条件，构建标准曲线。结果显示，选择1∶1000稀释的抗体，流速7 μL/min，反应时间4 min，构建标准曲线，可以达到较低的检测限。利用SPR生物传感器建立快速检测缓冲液中培氟沙星的方法，检测限为0.8 ng/mL，检测时间为4 min，为样品检测奠定基础。

关键词: 表面等离子共振; 生物传感器; 培氟沙星

Abstract: This experiment is to establish methods of detecting pefloxacin in HBS-EP buffer by SPR biosensor, which can provide a basis for sample detection. The pefloxacin-ovalbumin conjugate was covalently immobilized on CM5 chip and the antibody was mixed with pefloxacin of different concentrations to construct a standard curve. To obtain lower limit detection, the diluted antibody of 1000 times was injected at a flow rate of 7 μL/min for 4 min. It was shown from the experimental results that a rapid detection of pefloxacin in buffer using SPR biosensor had been established and the limit of detection was 0.8 ng/mL, the detection time was 4 min.

Key words: surface plasmon resonance; biosensor; pefloxacin

中图分类号:

TS207.53

郑晓华;魏星华;沈明浩. 表面等离子共振生物传感器检测培氟沙星的方法研究[J]. , 2010, 37(8): 233-235.

ZHENG Xiao-hua;WEI Xing-hua;SHEN Ming-hao. The Methods of Detecting Pefloxacin by Surface Plasmon Resonance Biosensor[J]. , 2010, 37(8): 233-235.

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