猪表皮生长因子基因的遗传变异及其与产仔性状的相关性分析

›› 2010, Vol. 37 ›› Issue (5): 147-150.

猪表皮生长因子基因的遗传变异及其与产仔性状的相关性分析

周利华1，肖石军1，杨广成2，陈从英1，丁能水1，高军1

（1.江西农业大学江西省动物生物技术重点开放实验室，南昌 330045;2.泰山学院生物科学与技术系，泰安 271021）

收稿日期:1900-01-01 修回日期:2010-04-16 出版日期:2010-05-20 发布日期:2010-05-20
通讯作者: 高军

Genetic Variation of the Porcine Epidermal Growth Factor Gene and its Association with Litter Size

ZHOU Li-hua1，XIAO Shi-jun1，YANG Guang-cheng2，CHEN Cong-ying1，DING Neng-shui1，GAO Jun1

(1.Jiangxi Provincial Key Laboratory for Animal Biotechnology, Jiangxi Agricultural University, Nanchang 330045, China;2.Department of Biological Science and Technology, Taishan University, Taian 271021, China)

Received:1900-01-01 Revised:2010-04-16 Online:2010-05-20 Published:2010-05-20
Contact: GAO Jun

摘要/Abstract

摘要： 本研究通过建立两次PCR扩增分型法，分析猪表皮生长因子（epidermal growth factor, EGF）基因第3内含子上的875 bp插入片段在13个中外不同繁殖性能的猪种中的遗传变异，以及EGF基因在白色杜洛克×二花脸资源家系180头F2母猪中与繁殖性状的相关性。结果表明，不同类型中国地方猪种与西方商业猪种在EGF基因位点上存在偏态分布。在资源家系中该位点对总产仔数、产活仔数和断奶活仔数无显著影响（P>0.05）；尽管BB基因型的产活仔数和断奶活仔数分别比AA型多1.74和0.46头，但BB型的个体太少，各基因型间的均值差异不显著（P>0.05）；虽然产活仔数的a值和d值分别达到0.87和1.24头，但该基因位点的加性效应和显性效应并不明显。因此，需要利用更多的SNP位点和更多样本进一步了解EGF基因与繁殖性状的关联性。

关键词: EGF基因; 产仔性状

Abstract: Genetic variation of an 875 bp insertion mutation located at intron 3 of epidermal growth factor (EGF) gene was analyzed by two-step PCR amplification in 13 Chinese and Western pig breeds having different reproduction ability, and genetic variation of this model with sow reproduction traits was also performed in 180 F2 sows from a White Duroc × Erhualian resource population. The results showed that the allele of this insertion mutation presented skew distribution in different types of Chinese indigenous pig breeds and Western commercial pig breeds. This mutation did not show any significant association with total number born，number born alive and number alive at weaning in this population，and the differences of mean value among genotypes also did not achieve significant level (P>0.05). Compared with individuals BB，individuals AA had 1.74 and 0.46 more number born alive and number alive at weaning，however there were only a few number of BB animals. Although the additive and dominant value of number born alive achieved 0.87 and 1.24，it did not achieve the significant level. So it was necessary to carry out SNP searching in the whole gene sequence and analyze the association with porcine reproduction traits in a large sample size.

Key words: pig; epidermal growth factor (EGF) gene; litter traits

中图分类号:

S828.8

周利华;肖石军;杨广成;陈从英;丁能水;高军. 猪表皮生长因子基因的遗传变异及其与产仔性状的相关性分析[J]. , 2010, 37(5): 147-150.

ZHOU Li-hua;XIAO Shi-jun;YANG Guang-cheng;CHEN Cong-ying;DING Neng-shui;GAO Jun. Genetic Variation of the Porcine Epidermal Growth Factor Gene and its Association with Litter Size[J]. , 2010, 37(5): 147-150.