›› 2010, Vol. 37 ›› Issue (5): 123-125.

• 遗传繁育 • 上一篇    下一篇

促黄体素对牛卵母细胞体外核成熟的影响

刘永杰1，夏怡2，金婧楠2，郭蕊2，刘云海2，郭勇2，李建红1，魏学良1   

  1. （1.西南大学动物科技学院, 重庆 400716； 2.北京农学院动物科学技术系， 北京 102206）
  • 收稿日期:1900-01-01 修回日期:1900-01-01 出版日期:2010-05-20 发布日期:2010-05-20
  • 通讯作者: 魏学良

Effect of Different Durations Treated by Luteinizing Hormone on Maturation in Vitro of Bovine Oocytes

LIU Yong-jie1, XIA Yi2, JIN Jing-nan2, GUO Rui2, LIU Yun-hai2, GUO Yong2, LI Jian-hong1, WEI Xue-liang1   

  1. （1.College of Animal Science and Technology, Southwest University, Chongqing 400716, China；2.Department of Animal Science & Technology, Beijing University of Agriculture, Beijing 102206, China）
  • Received:1900-01-01 Revised:1900-01-01 Online:2010-05-20 Published:2010-05-20
  • Contact: WEI Xue-liang

摘要： 为了研究促黄体素（luteinizing hormone,LH）在牛卵母细胞成熟培养体系中的时间依从性，试验通过向牛卵母细胞体外成熟培养的不同时间段添加LH来观察其核成熟的情况。首先用无LH的基础成熟液I和添加LH的成熟液Ⅱ分别培养，在成熟的不同时间点（3、6、9 h）用醋酸地衣红染色观察各自的生发泡破裂率。然后在体外成熟培养的不同时间段（0～3、0～6、0～9、0～24 h）添加LH，24 h后统计各自的第一极体率。结果表明，添加LH组在6 h的生发泡破裂率显著低于对照1组（P＜0.05），24 h添加LH组的第一极体率显著高于对照2组（P＜0.05）。因此，添加LH能延迟牛卵母细胞的核成熟，成熟培养中24 h全程添加LH较不添加组能显著提高核成熟率。

关键词: 促黄体素(LH); 牛; 卵母细胞; 体外成熟

Abstract: In this study, it was aimed to investigate the effect of different treatment durations of luteinizing hormone (LH) on maturation in vitro of bovine oocytes. The experiments were designed as following：first,the bovine cumulus oocytes complexes(COCs) were matured in vitro in medium with or without LH, the appropriate GVBD rate about it was recorded and analyzed to determine the time course of their maturation in vitro. Second,the bovine COCs were in vitro matured in medium with LH during different durations respectively (0 to 3 h,0 to 6 h,0 to 9 h and 0 to 24 h). After 24 h for in vitro maturation, their percentage of polar bodyⅠ(pbⅠ) was also analyzed. The GVBD rate in the group treated with LH for 6 h(30.19%±5.22％) was significantly lower than the control 1 group (48.42%±5.92%) (P＜0.05). Meanwhile, the percentage of polar bodyⅠ(pbⅠ) in group 4 treated by LH (81.05%±2.70％) was significantly higher than that of the control 2 group (65.89%±3.34％)(P＜0.05). Thus, it might be concluded that LH may improve the nuclear maturation of bovine oocytes while they are matured in vitro within 24 h.

Key words: luteinizing hormone (LH); bovine; oocytes; in vitro maturation

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