›› 2010, Vol. 37 ›› Issue (3): 131-136.

• 遗传繁育 • 上一篇    下一篇

德州驴心脏脂肪酸结合蛋白(H-FABP)基因的克隆及序列分析

李雷1， 孙玉江1，2， 柳林3， 闵令江1， 迟良1， 潘庆杰1   

  1. （1.青岛农业大学动物科技学院， 动物生殖发育与基因工程研究所， 青岛 266109；2.山东省东营市农业科学研究所， 东营 257091； 3.山东省烟台市动物疫病预防与控制中心， 烟台 264003）
  • 收稿日期:1900-01-01 修回日期:1900-01-01 出版日期:2010-03-20 发布日期:2010-03-20
  • 通讯作者: 潘庆杰

Cloning and Sequence Analysis of Dezhou Donkey Heart Fatty Acid-binding Protein(H-FABP) Gene

LI Lei1， SUN Yu-jiang1，2， LIU Lin3， MIN Ling-jiang1， CHI Liang1， PAN Qing-jie1   

  1. （1.Institute of Reproduction Development and Genetic Engineering，College of Animal Science and Technology，Qingdao Agricultural University，Qingdao 266109，China； 2.Institute of Agricultural Science of Dongying of Shandong Province，Dongying 257091，China； 3.Yantai Animal Disease Prevention and Control Center，Yantai 264003，China）
  • Received:1900-01-01 Revised:1900-01-01 Online:2010-03-20 Published:2010-03-20
  • Contact: PAN Qing-jie

摘要： 本试验对德州驴心脏脂肪酸结合蛋白(H-FABP)基因进行了克隆测序，并与GenBank中11个物种相应基因编码区核苷酸序列、氨基酸序列进行了比对分析，同时通过最小进化法（ME法）、邻接法（NJ法）和非加权组平均法（UPGMA法）对H-FABP基因编码区核苷酸序列进行了物种间分子系统进化树分析。结果表明，德州驴H-FABP基因由4个外显子和3个内含子组成，外显子1、2、3、4大小分别为73、173、102、57 bp，内含子1、2、3大小分别为3500、1980、1425 bp。CDS序列全长为405 bp，前体氨基酸数为134个；德州驴与马、野猪、人、牛、山羊、牦牛、大鼠、小鼠、红原鸡、绿头鸭、斑马鱼各物种在H-FABP基因编码区核苷酸序列上有较高的保守性，同源性大小分别为96.79%、91.36%、89.14%、88.89%、88.89%、88.64%、84.07%、83.46%、75.80%、74.81%、67.90%；3种方法构建的物种间分子系统进化树基本一致,并且3种分子进化树结果与动物学分类一致，表明H-FABP基因适合于构建不同物种间的系统进化树。

关键词: 德州驴; H-FABP基因; 克隆; 序列分析; 分子系统进化

Abstract: The Dezhou donkey heart fatty acid-binding protein (H-FABP) gene was cloned and compared the nucleotide sequence of coding region and amino-acid sequences with the homologous H-FABP genes in other eleven species retrieved from GenBank . The results showed that the Dezhou donkey H-FABP gene had four exons and three introns. The size of exon 1，exon 2，exon 3 and exon 4 were 73，173，102 and 57 bp respectively; while the size of intron 1，intron 2 and intron 3 were 3500，1981 and 1425 bp respectively. The size of CDS sequence of the Dezhou donkey H-FABP gene was 405 bp,which encoding 134 amino acids. The nucleotide sequences among different species were quite conservative. The homologies of the coding region of H-FABP genes between the Dezhou donkey and horse，Sus scrofa，human，cattle，goat，yak，rat，mouse，Gallus gallus，Anas platyrhynchos and zebra-fish were 96.79%，91.36%，89.14%，88.89%，88.89%，88.64%，84.07%，83.46%，75.80%，74.81% and 67.90% respectively. The phylogenetic trees were constructed by ME，NJ and UPGMA methods using the coding region DNA sequences of the H-FABP genes in all species. The results indicated that the molecular phylogenetic trees were basically coincident. This result of phylogenetic clustering was identical to the zoological classification system，indicating that the H-FABP gene was also fit to construct molecular phylogenetic tree among different species.

Key words: Dezhou donkey; heart fatty acid-binding protein gene; cloning; sequence analysis; molecular phylogenetic evaluation

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