猪胸膜肺炎放线杆菌血清3型间接血凝试验检测方法的建立

›› 2010, Vol. 37 ›› Issue (11): 143-145.

猪胸膜肺炎放线杆菌血清3型间接血凝试验检测方法的建立

赵彦宗，贺东生，苏丹萍

（华南农业大学兽医学院广东省人畜共患病重点实验室，广州 510642）

收稿日期:1900-01-01 修回日期:1900-01-01 出版日期:2010-11-20 发布日期:2010-11-20
通讯作者: 苏丹萍

Establishment of Indirect Haemagglutination Assays for Detection of Antibodies against Actinobacillus pleuropneumoniae Serotype 3

ZHAO Yan-zong, HE Dong-sheng,SU Dan-ping

（Guangdong Key Laboratory of Zoonosis, College of Veterinary Medicine, South China Agricultural University, Guangzhou 510642, China）

Received:1900-01-01 Revised:1900-01-01 Online:2010-11-20 Published:2010-11-20
Contact: SU Dan-ping

摘要/Abstract

摘要： 本试验用分离纯化的猪胸膜肺炎放线杆菌（APP）3型荚膜多糖（CPS），致敏双醛化处理的绵羊血红细胞，建立了APP的血清学诊断方法，结果表明，该方法特异性强，仅与APP 3型阳性血清反应，可以用其进行APP疫苗免疫后抗体检测，该诊断方法简单、快速，适用于临床疾病的诊断。

关键词: 猪胸膜肺炎放线杆菌; 荚膜多糖; 间接血凝试验

Abstract: The indirect hemagglutination assays was obtained by sensitization of sheep red blood cell with the separated and purifitied capsular polysaccharide (CPS) of Actinobacillus pleuropneumoniae serotype 3. The result showed that the speciality of the indirect hemagglutination assays was good. It could only agglutinate the same type positive antibody and could be used to detect Actinobacillus pleuropneumoniae serotype 3 antibody. Simple fast economic way to test Actinobacillus pleuropneumoniae antibody with the indirect hemagglutination assays. It is a valid means to diagnose and prevent Actinobacillus pleuropneumoniae.

Key words: Actinobacillus pleuropneumoniae; capsular polysaccharide; indirect haemagglutination assays

中图分类号:

S852.61

赵彦宗;贺东生;苏丹萍. 猪胸膜肺炎放线杆菌血清3型间接血凝试验检测方法的建立[J]. , 2010, 37(11): 143-145.

ZHAO Yan-zong;HE Dong-sheng;SU Dan-ping. Establishment of Indirect Haemagglutination Assays for Detection of Antibodies against Actinobacillus pleuropneumoniae Serotype 3[J]. , 2010, 37(11): 143-145.

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