›› 2009, Vol. 36 ›› Issue (5): 123-126.

• 遗传繁育 • 上一篇    下一篇

利用SRAP分子标记对北极狐遗传多样性的研究

张敏1,2, 白秀娟1   

  1. (1.东北农业大学动物科技学院, 哈尔滨 150030; 2.辽宁医学院 动物科技学院, 锦州 121001)
  • 收稿日期:1900-01-01 修回日期:1900-01-01 出版日期:2009-05-20 发布日期:2009-05-20
  • 通讯作者: 白秀娟

SRAP Use of Molecular Markers of Genetic Diversity of the Arctic fox Preliminary Study

ZHANG Min1,2,BAI Xiu-juan1   

  1. (1.Northeast Agricultural University, Institute of Animal Science and Technology, Harbin 150030, China;2.Liaoning College of Medicine, Institute of Animal Science and Technology, Jinzhou 121001, China)
  • Received:1900-01-01 Revised:1900-01-01 Online:2009-05-20 Published:2009-05-20
  • Contact: BAI Xiu-juan

摘要: 相关序列多态性(SRAP)是近年来发展起来的一种新型分子标记技术,具有稳定、简便、中等产率、高共显性等优点。本试验利用北极狐的基因组作为模版,首次把SRAP方法引入到哺乳动物中进行分析,对北极狐SRAP反应条件进行了优化,产物用非变性的聚丙烯酰胺凝胶分离,得到的结果清晰、稳定、多态性高,结果用NTSYS-pcversion 2.1软件对北极狐群体的遗传多样进行了初步的分析。

关键词: 北极狐; SRAP标记

Abstract: Related sequence polymorphism (SRAP) in recent years developed a new type of molecular marker technology, with a stable, simple, middle-yield, high-codominant advantages. In this study the use of the Arctic fox’s genome as a template for the first time the SRAP approach to the introduction of mammals in the analysis of the Arctic fox SRAP reaction conditions are optimized, the product of non-denatured polyacrylamide gel separation, results were clear, stable and high-band polymorphism, with the results of NTSYS-pcversion 2.1 software on the Arctic fox population of the genetic diversity of a preliminary analysis.

Key words: Arctic fox; SRAP marker

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