早孕因子的生物学特性及其在动物繁殖领域中的应用

›› 2009, Vol. 36 ›› Issue (4): 122-124.

早孕因子的生物学特性及其在动物繁殖领域中的应用

权凯1,2，张改平2

（1.郑州牧业工程高等专科学校，郑州 450011；2.河南省免疫学重点实验室，郑州 450004）

收稿日期:1900-01-01 修回日期:1900-01-01 出版日期:2009-04-20 发布日期:2009-04-20
通讯作者: 张改平

The Biological Characteristics and Application in Animal Reproduction Field of EPF

QUAN Kai1,2, ZHANG Gai-ping2

(1.Zhengzhou College of Animal Husbandry Engineering, Zhengzhou 450011, China;2.He’nan Provincial Key Laboratory for Animal Immunology, Zhengzhou 450004, China)

Received:1900-01-01 Revised:1900-01-01 Online:2009-04-20 Published:2009-04-20
Contact: ZHANG Gai-ping

摘要/Abstract

摘要： 早孕因子（early pregnancy factor，EPF）最初发现于孕鼠血清，是妊娠哺乳动物血清中存在的一种具有免疫抑制和生长调节作用的妊娠相关蛋白。EPF的氨基酸序列包括102个氨基酸残基，与伴侣蛋白10（chaperonin 10，Cpn10）高度同源，是Cpn10在细胞外发挥的效应，对温度、酸碱度的耐受性较强，无动物及品种特异性。其生物学特性主要体现在抑制免疫和生长调节两方面：①EPF具有抑制母体的免疫反应而使精子、胚胎免受免疫排斥的作用，也是目前最早确认妊娠的生化标志之一；②EPF能促进肿瘤细胞的生长，也能促进烧伤、溃疡等伤口的愈合。由于EPF能增强抗淋巴细胞血清抑制T细胞花环的形成，因此，玫瑰花环抑制试验是检测EPF活性的经典方法。EPF在动物繁殖领域可用于动物的早期、超早期妊娠诊断，妊娠中断的检测，胚胎移植后的成活情况检测，在评价雄性繁殖力和繁殖免疫疾病的治疗等方面有着重要价值。

关键词: 早孕因子; 伴侣蛋白10; 玫瑰花环抑制试验

Abstract: Early pregnancy factor (EPF) was first found in the serum of pregnant rats, is one kind pregnancy-associated protein which exists in the pregnancy mammal blood serum and has immunosuppressive and growth regulation effect. The EPF amino acid sequence including 102 amino acid residues, with the chaperonin l0 homology, is the extracellular effects of Cpn10, which temperature and pH value tolerance is strong, and don’t has animals and species-specific. Its biology characteristic mainly manifests in the suppression immunity and the growth adjusts. Firstly, EPF can inhibit the mother’s immune response to prevent sperm, embryos from immune rejection, and is also the most early confirms one of pregnancy biochemistry symbols at present. Secondly, EPF can promote tumor cell growth as well as promote burns, ulcers, such as wound healing. Since the EPF can enhance anti-lymphocyte serum inhibited T cell rosette formation, therefore, the rosette inhibition test is the classic method of detecting EPF activity. EPF can be used in animal reproduction domain to diagnose animal’s early time, super early pregnancy, pregnancy interrupt examination, the survival state after embryo transfer, has an important value in the evaluation of male fertility and reproduction and the treatment of diseases.

Key words: early pregnancy factor; chaperonin 10; rosette inhibition test

中图分类号:

S821.3

权凯;;张改平. 早孕因子的生物学特性及其在动物繁殖领域中的应用[J]. , 2009, 36(4): 122-124.

QUAN Kai;;ZHANG Gai-ping. The Biological Characteristics and Application in Animal Reproduction Field of EPF[J]. , 2009, 36(4): 122-124.