中国奶牛PrP基因型分析

›› 2009, Vol. 36 ›› Issue (3): 110-111.

中国奶牛PrP基因型分析

张太翔，田国华，田国宁，张金玲，韩亮，张丽萍

（潍坊出入境检验检疫局，山东 261041）

收稿日期:1900-01-01 修回日期:1900-01-01 出版日期:2009-03-20 发布日期:2009-03-20

Analysis of Genotype of PrP Gene in China Cow

ZHANG Tai-xiang, TIAN Guo-hua, TIAN Guo-ning, ZHANG Jin-ling, HAN Liang, ZHANG Li-ping

(Weifang Entry-Exit Inspection and Quarantine Bureau of People’s Republic of China, Weifang 261041, China)

Received:1900-01-01 Revised:1900-01-01 Online:2009-03-20 Published:2009-03-20

摘要/Abstract

摘要： 根据已报道奶牛朊蛋白(PrP)基因序列设计引物,采用PCR法扩增了28头黑白花奶牛的PrP基因。序列测定及分析结果表明，所克隆的奶牛PrP基因片段为677 bp,编码225个氨基酸的前体蛋白。对其136、154、171位点氨基酸多态性分析结果发现，对TSE中度易感的ARQ型占检测奶牛的82.9%，对TSE抗性的ARR型占7.1%，对TSE高度易感的VRQ型占10%。这说明中国奶牛的基因型对TSE中度易感，为防制TSE的发生提供基础数据。

关键词: 黑白花奶牛; 朊病毒基因型; 多态性分析

Abstract:

We got the PrP gene of 28 black and white cows by PCR using the primers designed according to the reported cow PrP gene. Sequence analysis showed the segment of cow PrP gene were 677 bp, coding 225 amino acids. The analysis of polymorphism of 136,154,171 amino acid site of prion protein revealed the genotype of ARQ associated with an intermediate susceptibility to TSE was present in 82.9% of cow examined, the genotype of ARR that associated with the resistance to TSE was present in 7.1% of cow examined, the genotype of VRQ associated with high susceptibility to TSE was present in 10% of cow examined. It suggested that the cow in our country was intermediate susceptibility to TSE, which could support the basic data to prevent and control the TSE in our country.

Key words: blace and white cow; genotype of PrP gene; polymorphism analysis

中图分类号:

S852.65⁺9.7

张太翔;田国华;田国宁;张金玲;韩亮;张丽萍. 中国奶牛PrP基因型分析[J]. , 2009, 36(3): 110-111.

ZHANG Tai-xiang;TIAN Guo-hua;TIAN Guo-ning;ZHANG Jin-ling;HAN Liang;ZHANG Li-ping. Analysis of Genotype of PrP Gene in China Cow[J]. , 2009, 36(3): 110-111.