化学发光直接竞争免疫分析法检测猪尿中莱克多巴胺残留

›› 2009, Vol. 36 ›› Issue (12): 200-203.

化学发光直接竞争免疫分析法检测猪尿中莱克多巴胺残留

高彬文^1，2，张素霞²，梁永红¹，刑保松^1

（1.河南农业科学院畜牧兽医研究所，郑州 450002； 2.中国农业大学动物医学院，北京 100193）

收稿日期:1900-01-01 修回日期:1900-01-01 出版日期:2009-12-20 发布日期:2009-12-20
通讯作者: 张素霞

Determination of Ractopamine Residues in Swine Urine by Direct Competitive Chemiluminescence Immunoassay

GAO Bin-wen^1，2, ZHANG Su-xia², LIANG Yong-hong¹, XING Bao-song¹

(1.Institute of Animal Husbandry and Veterinary Research Science, Henan Agriculture Science, Zhengzhou 450002, China; 2.College of Veterinary Medicine, China Agricultural University, Beijing 100193, China)

Received:1900-01-01 Revised:1900-01-01 Online:2009-12-20 Published:2009-12-20
Contact: ZHANG Su-xia

摘要/Abstract

摘要： 本试验研究建立了猪尿中莱克多巴胺（Ractopamine, RAC）残留的直接竞争化学发光免疫检测方法。合成了莱克多巴胺和辣根过氧化物酶的偶联物（RAC-HRP），方法的检测限为0.09 ng/mL，空白猪尿中添加浓度为0.5、10和100 ng/mL RAC时，检测范围为0.3～157.0 ng/mL；回收率为76.3%～87.6%，批内变异系数为10.0%～12.2%，批间变异系数为14.2%～15.2%。

关键词: 化学发光免疫分析; 莱克多巴胺; 猪尿

Abstract:

A method was developed for the determination of RAC residues in swine urine by direct competitive chemiluminescence immunoassay (CLIA). The limit of detection was 0.09 ng/mL and the detection ranged from 0.3 ng/mL to 157.0 ng/mL. At the fortified levels of 0.5 ng/mL, 10 ng/mL and 100 ng/mL, mean recoveries ranged from 76.3% to 87.6%. The intra-assay variations were 10.0% to 12.2% and the inter-assay variations were 14.2% to 15.2%.

Key words: chemiluminescence immunoassay; Ractopamine; swine urine

中图分类号:

S859.84

高彬文;张素霞;梁永红;刑保松. 化学发光直接竞争免疫分析法检测猪尿中莱克多巴胺残留[J]. , 2009, 36(12): 200-203.

GAO Bin-wen;ZHANG Su-xia;LIANG Yong-hong;XING Bao-song. Determination of Ractopamine Residues in Swine Urine by Direct Competitive Chemiluminescence Immunoassay[J]. , 2009, 36(12): 200-203.

[1]	李丹妮, 黄华, 张婧, 王学生, 顾依雯. 3种β₂-受体激动剂荧光免疫层析试纸条的制备及特性研究[J]. 《中国畜牧兽医》, 2017, 44(8): 2437-2442.
[2]	王文珺, 李阳, 叶云锋, 江海洋. 生物样本中莱克多巴胺检测方法的研究进展[J]. , 2015, 42(1): 140-146.
[3]	郑百芹;李爱军;张建民;董李学;蒙君丽;肖琎. 液相色谱-串联质谱法测定猪肉中3种β-受体激动剂类药物残留[J]. , 2012, 39(3): 224-227.
[4]	高彬文;张素霞;梁永红;邢保松. 化学发光免疫分析法检测猪尿中莱克多巴胺残留[J]. , 2009, 36(8): 27-29.
[5]	左晓磊;邱伟;韩爱云;邱建琴. 莱克多巴胺在畜禽生产中的应用及残留[J]. , 2007, 34(9): 12-14.
[6]	于洪侠;杨曙明. 两种不同偶联比的莱克多巴胺免疫原免疫效果比较[J]. , 2007, 34(4): 148-149.
[7]	温涛;赵建增;罗胜军;高荣;高英杰. 化学发光免疫分析在兽医诊断中的应用[J]. , 2007, 34(10): 73-75.
[8]	田慧丽;刘孟洲. 甘肃地区猪肉中莱克多巴胺残留量的检测分析[J]. , 2007, 34(10): 136-138.