抗水牛伊氏锥虫变异表面糖蛋白抗原单克隆抗体的研制

›› 2009, Vol. 36 ›› Issue (10): 72-74.

抗水牛伊氏锥虫变异表面糖蛋白抗原单克隆抗体的研制

曾晓飞，陈汉忠，韦英益，何木荣，李晓栩

（广西大学动物科技学院，南宁 530005）

收稿日期:1900-01-01 修回日期:1900-01-01 出版日期:2009-10-20 发布日期:2009-10-20
通讯作者: 陈汉忠

Preparation of Monoclonal Antibody against Variant Surface Glucoprotein Antigen of Trypanosoma evansi

ZENG Xiao-fei, CHEN Han-zhong, WEI Ying-yi, HE Mu-rong, LI Xiao-xu

(College of Animal Science and Technology, Guangxi University, Nanning 530005, China)

Received:1900-01-01 Revised:1900-01-01 Online:2009-10-20 Published:2009-10-20
Contact: CHEN Han-zhong

摘要/Abstract

摘要： 用纯化的伊氏锥虫变异表面糖蛋白（variant surface glucoprotein, VSG）免疫BALB/c小鼠，取免疫小鼠脾细胞与SP2/0骨髓瘤细胞进行融合，经过3次克隆和间接ELISA方法筛选，获得3D7、5B9 2株稳定分泌单克隆抗体的杂交瘤细胞株，用间接ELISA方法检测杂交瘤细胞培养液上清效价和小鼠腹水效价，其中细胞培养上清效价分别为1∶6400和1∶12800，腹水效价分别为1∶105和1∶106。单抗的亚型鉴定结果表明，3D7、5B9分泌的抗体都为IgG1亚类κ链。

关键词: 变异表面糖蛋白; 伊氏锥虫; 单克隆抗体

Abstract: The BALB/c mice were immunized with variant surface glucoprotein of Trypanosoma evansi. The spleen lymph cells were fused with mouse SP2/0 myeloma cells, two hybridoma cell lines 3D7 and 5B9 secreting monoclonal antibodies against variant surface glucoprotein were determined by indirect ELISA. Their titers in cell culture supernatant were 1∶6400 and 1∶12800, and titers of ascites were 1∶105 and 1∶106, both 3D7 and 5B9 belongs to IgG1 with κ chain.

Key words: variant surface glucoprotein; Trypanosoma evansi; monoclonal antibody

中图分类号:

S859.79

曾晓飞;陈汉忠;韦英益;何木荣;李晓栩. 抗水牛伊氏锥虫变异表面糖蛋白抗原单克隆抗体的研制[J]. , 2009, 36(10): 72-74.

ZENG Xiao-fei;CHEN Han-zhong;WEI Ying-yi;HE Mu-rong;LI Xiao-xu. Preparation of Monoclonal Antibody against Variant Surface Glucoprotein Antigen of Trypanosoma evansi[J]. , 2009, 36(10): 72-74.

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