蝉抗菌肽基因密码子优化及其毕赤酵母表达载体构建

›› 2009, Vol. 36 ›› Issue (10): 44-46.

蝉抗菌肽基因密码子优化及其毕赤酵母表达载体构建

刘德辉，黄毓茂，徐蕙，焦颖

(华南农业大学兽医学院，广州 510642)

收稿日期:1900-01-01 修回日期:1900-01-01 出版日期:2009-10-20 发布日期:2009-10-20
通讯作者: 黄毓茂

Codon Optimization of Cicada Antimicrobial Peptide Gene and its Expression Vector Construction in Pichia pastoris

LIU De-hui, HUANG Yu-mao, XU Hui, JIAO Ying

(College of Veterinary Medicine, South China Agricultural University, Guangzhou 510642, China)

Received:1900-01-01 Revised:1900-01-01 Online:2009-10-20 Published:2009-10-20
Contact: HUANG Yu-mao,

摘要/Abstract

摘要： 根据毕赤酵母（Pichia pastoris）密码子的偏嗜性，通过SOEing法合成改造后的抗菌肽Cryptonin基因片段。基因克隆到pGAPZα-A质粒中，获得分泌型重组酵母表达载体pGAPZα-A-Cryptonin。经酶切分析、PCR和测序鉴定证明载体构建成功。表达载体的正确构建为Cryptonin的高效表达和生物学活性研究奠定基础。

关键词: Cryptonin; 密码子优化; pGAPZα-A载体; 载体构建

Abstract: According to the partiality codon of Pichia pastoris, the antibacterial peptide Cryptonin gene was designed and synthesized. The modified antibacterial peptide gene was cloned into the pGAPZα-A vector to construct the recombinant expression vector pGAPZα-A-Cryptonin. Then the plasmid was identified by restriction enzyme digestion, PCR amplification and sequencing. The successfully constructed plasmid is the background for the studies on high expression and bioactivities of Cryptonin.

Key words: Cryptonin; codon optimization; pGAPZα-A vector; vector construction

中图分类号:

刘德辉;黄毓茂;徐蕙;焦颖. 蝉抗菌肽基因密码子优化及其毕赤酵母表达载体构建[J]. , 2009, 36(10): 44-46.

LIU De-hui;HUANG Yu-mao;XU Hui;JIAO Ying. Codon Optimization of Cicada Antimicrobial Peptide Gene and its Expression Vector Construction in Pichia pastoris[J]. , 2009, 36(10): 44-46.

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