鸡胚蛋重比作为评价传染性支气管炎病毒致侏儒化指标的研究

›› 2009, Vol. 36 ›› Issue (1): 86-87.

鸡胚蛋重比作为评价传染性支气管炎病毒致侏儒化指标的研究

程亚辉，崔保安，李新生，李双亮，冯利霞，高文明

河南农业大学牧医工程学院，郑州 450002

收稿日期:1900-01-01 修回日期:1900-01-01 出版日期:2009-01-20 发布日期:2009-01-20

Study on the EE Ratio as an Indicator of Dwarfism Induced by Infectious Bronchitis Virus

CHENG Yahui, CUI Baoan, LI Xinsheng, LI Shuangliang, FENG Lixia, GAO Wenming

College of Animal Husbandry and Veterinary Science, Henan Agricultural University,Zhengzhou 450002,China

Received:1900-01-01 Revised:1900-01-01 Online:2009-01-20 Published:2009-01-20

摘要/Abstract

摘要： 在鸡胚上接种鸡传染性支气管病毒（M41、H120、HN99株）后6 d内，每天称量胚体和各自种蛋的重量，计算胚体/种蛋重量的比值（EE值）。EE值与未接种病毒的对照组鸡胚相比，计算出最小感染量，EE指数通过接种过IBV的鸡胚EE值和对照组EE值相比计算出来。结果表明，在接种M41株和HN99株后第4天EE值与对照组差异显著，在接种H120株后第5天EE值与对照组差异显著，可初步判定是被传染性支气管炎病毒感染，这种方法可更快更有效的观察胚体损伤程度，尤其适用于需经传代才能看到矮小化现象的野毒株。

关键词: 传染性支气管炎病毒（IBV）; 鸡胚蛋重比（EE值）; EE指数

Abstract: Avian infectious bronchitis virus (M41,H120,HN99) were inoculated in chicken embryos.The eggs and the respective embryos were weighed and embryo:egg weight (EE) ratios were calculated within 6 days post inoculation.The EE ratios were compared with the uninoculated control embryos and EID50 could be calculated objectively. EE indices were also calculated by dividing the EE ratios of inoculated chicken embryos by the mean EE ratio of uninoculated controls. The results indicated that values of M41 and HN99 differ significantly from the respective fourth day EE ratio of uninoculated control embryos, and H120 differ significantly from the respective fifth day EE ratio of uninoculated control embryos, then the preliminary determination is to be infectioned by the infectious bronchitis virus. This method would be useful to observe embryo lesions especially in field strains of infectious bronchitis virus, which does not cause observable dwarfing until several embryo passages.

Key words: infectious bronchitis virus; EE ratio; EE indices

程亚辉;崔保安;李新生;李双亮;冯利霞;高文明. 鸡胚蛋重比作为评价传染性支气管炎病毒致侏儒化指标的研究[J]. , 2009, 36(1): 86-87.

CHENG Yahui;CUI Baoan;LI Xinsheng;LI Shuangliang;FENG Lixia;GAO Wenming. Study on the EE Ratio as an Indicator of Dwarfism Induced by Infectious Bronchitis Virus[J]. , 2009, 36(1): 86-87.

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