关键词: 狮头鹅; α-干扰素; 克隆; 序列分析

Abstract: With nested transcription polymerrase chain reaction, the interferon-α gene fragment of shitou goose (d-Go-IFN-α) was amplified from genome DNA extracted from peripheral blood cells (PBCs). At the same time, the total RNA was extracted from peripheral blood lymphocyte (PBLs) stimulated with Newcasttle disease virus(NDV) in vivo, and transcription polymerrase chain reaction(RT-PCR) was used to amplify the interferon-α gene fragment of shitou goose (m-Go-IFN-α), then both of the amplified fragments were cloned into pGEM-T easy vector and sequenced. The results showed that both of the d-Go-IFN-α and m-Go-IFN-α were both consisted of 576 necleotides, coding for 192 amino acids. Compared with the nucleotide sequences of d-Go-IFN-α and m-Go-IFN-α, a variation A→G at the 155th site was observed, and the 52th amino acid was mutated from M to R accordingly, which implied that there was single nucleotde polymorphism in IFN-α sequence.of Shitou goose. The homolony of nucleotide and the deduced amino acid sequence among different poultry breeds were 41.2 % to 98.3% and 13.0% to 96.4% respectively. The secondary structure of the IFN-α were almost the same among different goose breeds. 

Key words: Shitou goose; interferon-α; gene cloning; sequence ananlysis