›› 2008, Vol. 1 ›› Issue (9): 27-29.

• 生物技术 • 上一篇    下一篇

猪链球菌2型对PK-15细胞的黏附动力学

姜成刚1,刘荻萩2,蔡雪辉1   

  1. 1.中国农业科学院哈尔滨兽医研究所,哈尔滨 150001;2.东北农业大学,哈尔滨 150030 
  • 收稿日期:1900-01-01 修回日期:1900-01-01 出版日期:2008-09-20 发布日期:2008-09-20

Adhesion Kinesics of Streptococcus suis Type 2 to PK-15 Cells

JIANG Chenggang1, LIU Diqiu2, CAI Xuehui1
  

  1. 1.Harbin Veterinary Research Institute, CAAS,Harbin 150001,China;2.Northeast Agriculture University, Harbin 150030,China
  • Received:1900-01-01 Revised:1900-01-01 Online:2008-09-20 Published:2008-09-20

摘要: 为研究携带不同毒力因子的猪链球菌2型对细胞侵袭作用的差异,本实验室从全国各地分离100多株猪链球菌,PCR鉴定出10株链球菌2型,其中携带毒力因子菌株为7-4[MRP+EF+]、8-3[MRP+EF-]、ZH-1[MRP-EF+],另外选择1株LN-2 [MRP-EF-]与PK15细胞进行黏附动力学试验,结果表明MPR毒力因子与菌株对PK-15细胞黏附具有密切相关性。

关键词: 猪链球菌2型; PK-15细胞; 黏附动力学

Abstract: The objective of this paper was to study the different invasiveness among streptococcus suis type 2 which carried various virulence factors,100 strains of S suis were isolated from all over the country. 10 strains of them were S suis type 2 by PCR identification, strains with virulence factors were selected to explore the adhevence of them to PK-15 cells.7-4[MRP+EF+],8-3[MRP+EF-],ZH-1[MRP-EF+],and control strains LN-2 [MRPEF] were chose in adhesion kinesics test to PK-15 cells.The results indicated that MRP factor was closely related to adhesion of S suis type 2 to PK-15 cells.

Key words: streptococcus suis; PK-15 cells; adhesion kinesics

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