荧光原位杂交技术在动物肠道微生物定量中的应用

›› 2008, Vol. 1 ›› Issue (12): 53-55.

荧光原位杂交技术在动物肠道微生物定量中的应用

奚晓琦^1，2，王加启¹，邓露芳¹，李旦¹，卜登攀¹，魏宏阳¹

1.中国农业科学院北京畜牧兽医研究所动物营养学国家重点实验室，北京 100193；2.东北农业大学，哈尔滨 150030

收稿日期:1900-01-01 修回日期:1900-01-01 出版日期:2008-12-20 发布日期:2008-12-20

Fluorescence in Situ Hybridization and its Applications in Quantification of Intestinal Microbe

XI Xiaoqi1,2,WANG Jiaqi1,DENG Lufang1,LI Dan1,BU Dengpan1,WEI Hongyang1

1.Institute of Animal Science of Chinese Academy of Agricultural Sciences, State Key Laboratory of Animal Nutrition,Beijing 100193, China;2.Northeast Agriculture University, Harbin 150030, China

Received:1900-01-01 Revised:1900-01-01 Online:2008-12-20 Published:2008-12-20

摘要/Abstract

摘要： 以16S rRNA为靶序列的寡核苷酸探针荧光原位杂交技术(fluorescence in situ hybridization，FISH)已广泛应用于分析环境中复杂的微生物群落构成。作者简要介绍了荧光原位杂交技术的原理和操作方法，对其在动物肠道中微生物鉴定和计数上的应用进行了概述，最后探讨了FISH技术的局限性及其应用前景。

关键词: 荧光原位杂交; 16S rRNA寡核苷酸探针; 肠道微生物

Abstract: Fluorescence in situ hybridization(FISH) has been widely applied in analyzing the complex compositions of microbial community. This paper introduced the mechanism of fluorescence in situ hybridization technology and its application in quantification of intestinal microbe.

Key words: fluorescence in situ hybridization; 16S rRNAtargeted oligonucleotide probes; intestinal microbe

奚晓琦;王加启;邓露芳;李旦;卜登攀;魏宏阳. 荧光原位杂交技术在动物肠道微生物定量中的应用[J]. , 2008, 1(12): 53-55.

XI Xiaoqi;;WANG Jiaqi;DENG Lufang;LI Dan;BU Dengpan;WEI Hongyang. Fluorescence in Situ Hybridization and its Applications in Quantification of Intestinal Microbe[J]. , 2008, 1(12): 53-55.