关键词: 猪转移因子; MTT法; 中性粒细胞

Abstract: The requisite swine transfer factor (TF) was prepared by relative standards and was intramuscularly injected to a range of hybrid shepherd dogs to determine the number and bactericidal activity of neutrophilic granulocyte in canine peripheral blood by phagocytosis MTT. And the best condition for MTT to be applied into determining the ability of phagocytosis of neutrophilic granulocyte against Escherichia coli in canine peripheral blood were explored as 1.3×106 cells/ml of neutrophilic granulocytes and 6×105 units /ml had been mixed and incubated together for 2 hours followed by adding MTT and incubating for another 4 hours. The results of experiments in vitro showed that swine TF at 0.052～1.56 mg/ml markedly promoted the phagocytosis of neutrophilic granulocyte against E coli, with the strongest effect to the phagocytosis of neutrophilic granulocyte against E coli at 1.56 mg/ml; The number of neutrophilic granulocytes in canine peripheral blood climbed to the summit and the effect of phagocytosis of neutrophilic granulocyte against E coli was the strongest on the 2nd day after the dogs were intramuscularly injected with swine TF.

Key words: swine transfer factor; MTT assay; neutrophilic granulocytes