›› 2007, Vol. 34 ›› Issue (9): 58-60.

• 生物技术 • 上一篇    下一篇

抗菌肽Cecropin P1基因真核表达载体的构建

李建霞1,2,苗向阳2，丁兆忠2，任慧英1, 2, 于忠娜1,2
   

  1. 1.莱阳农学院动物科技学院,城阳 266109；2.中国农业科学院北京畜牧兽医研究所,动物营养学国家重点实验室, 北京 100094
  • 收稿日期:1900-01-01 修回日期:1900-01-01 出版日期:2007-09-20 发布日期:2007-09-20

Construction of Eukaryotic Expression Vector of Antimicrobial Peptide Gene Cecropin P1

LI Jianxia1,2, MIAO Xiangyang2, DING Zhaozhong2 , REN Huiying1,2, YU Zhongna1,2   

  1. 1.Animal Science and Technology Department of Laiyang Agriculture College, Chengyang 266109，China; 2.Institute of Animal Sciences，Chinese Academy of Agriculture Sciences, State Key Laboratory of Animal Nutrition, Beijing 100094,China
  • Received:1900-01-01 Revised:1900-01-01 Online:2007-09-20 Published:2007-09-20

摘要： 选择大肠杆菌偏爱密码子，人工设计合成3条抗菌肽Cecropin P1基因寡核苷酸片段，通过PCR扩增得到抗菌肽Cecropin P1基因的全序列，并将其克隆到T-easy载体上，经双酶切重组于真核表达质粒pcDNA3.1（+）上，经酶切鉴定和序列分析，抗菌肽Cecropin P1基因表达载体构建成功。抗菌肽Cecropin P1基因表达载体的成功构建，为进一步研究其抗菌活性、抗菌机理及应用等打下基础。

关键词: Cecropin P1; 表达载体构建; 抗菌肽

Abstract: Three gene fragments were designed and synthesized according to the preference of E.coli.The gene of Cecropin P1 was amplified by PCR. The target gene fragment modified was cloned into vector T-easy, and then inserted into expression plasmid pcDNA3.1(+),the expression vector construction was completed. It was confirmed that the rombinant plasmid was successfully constructed by restriction endonuclease analysis and DNA sequencing, This may lay a foundation in research of antimicobial activities and antimicrobial mechanism in the gene of Cecropin P1.

Key words: Cecropin P1; construction of expression vector; antimicrobial peptide

中图分类号: 

京ICP备10030713号-19
版权所有 © 2019 中国农业科学院北京畜牧兽医研究所
地址：北京市海淀区圆明园西路农大南路中国农业科学院北京畜牧兽医研究所　邮编：100193　
电话：010-62811226，62810371，62816020　E-mail：zgxmsy@caas.cn
本系统由北京玛格泰克科技发展有限公司设计开发