›› 2007, Vol. 34 ›› Issue (9): 58-60.
• 生物技术 • 上一篇 下一篇
李建霞1,2,苗向阳2，丁兆忠2，任慧英1, 2, 于忠娜1,2
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LI Jianxia1,2, MIAO Xiangyang2, DING Zhaozhong2 , REN Huiying1,2, YU Zhongna1,2
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摘要： 选择大肠杆菌偏爱密码子，人工设计合成3条抗菌肽Cecropin P1基因寡核苷酸片段，通过PCR扩增得到抗菌肽Cecropin P1基因的全序列，并将其克隆到T-easy载体上，经双酶切重组于真核表达质粒pcDNA3.1（+）上，经酶切鉴定和序列分析，抗菌肽Cecropin P1基因表达载体构建成功。抗菌肽Cecropin P1基因表达载体的成功构建，为进一步研究其抗菌活性、抗菌机理及应用等打下基础。
关键词: Cecropin P1; 表达载体构建; 抗菌肽
Abstract: Three gene fragments were designed and synthesized according to the preference of E.coli.The gene of Cecropin P1 was amplified by PCR. The target gene fragment modified was cloned into vector T-easy, and then inserted into expression plasmid pcDNA3.1(+),the expression vector construction was completed. It was confirmed that the rombinant plasmid was successfully constructed by restriction endonuclease analysis and DNA sequencing, This may lay a foundation in research of antimicobial activities and antimicrobial mechanism in the gene of Cecropin P1.
Key words: Cecropin P1; construction of expression vector; antimicrobial peptide
中图分类号:
Q782
李建霞;苗向阳;丁兆忠;任慧英;于忠娜; . 抗菌肽Cecropin P1基因真核表达载体的构建 [J]. , 2007, 34(9): 58-60.
LI Jianxia;MIAO Xiangyang; DING Zhaozhong;REN Huiying;YU Zhongna;. Construction of Eukaryotic Expression Vector of Antimicrobial Peptide Gene Cecropin P1[J]. , 2007, 34(9): 58-60.
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