›› 2007, Vol. 34 ›› Issue (8): 75-77.

• 疾病防治 • 上一篇    下一篇

广西野猪猪瘟病毒感染情况的调查报告

陈凤莲,马玲,潘汉世,黄红梅,孙建华,陈忠伟,吴健敏
  

  1. 广西兽医研究所,南宁 530001
  • 收稿日期:1900-01-01 修回日期:1900-01-01 出版日期:2007-08-20 发布日期:2007-08-20

A Survey of Wild Boars in Guangxi for Infection of Classical Swine Fever

CHEN Fenglian, MA Ling, PAN Hanshi, HUANG Hongmei, SUN Jianhua,
CHEN Zhongwei, WU Jianmin
  

  1. Guangxi Veterinary Research Institute,Nanning 530001,China
  • Received:1900-01-01 Revised:1900-01-01 Online:2007-08-20 Published:2007-08-20

摘要: 作者报道采用RT-PCR和ELISA方法检测猪瘟病毒在野猪身上的感染情况。采集广西5个地市28份野猪脾淋组织作病毒检测,设计针对猪瘟(classical swine fever, CSF)病毒的特异性引物CSFVE2,进行RT-PCR检测,所检样品结果全呈猪瘟阴性;同时,运用酶联免疫吸附测定(ELISA)试剂盒检测以上样品,结果也都呈阴性。这一结果初步表明所检测的广西5个地区的野猪没有感染猪瘟病毒。

关键词: 猪瘟病毒; 野猪; RT-PCR; ELISA

Abstract: RT-PCR and ELISA detected CSFV in wild boars in this article. A pair of specific primers were designed and synthesized, then RT-PCR was used to detect 28 spleens and lymph tissues of wild boars from5 cities in Guangxi province, and the results were all positive; meanwhile, ELISA was used to detected these samples ,the results were also all positive. This result indicates that wild boars from 5cities in Guangxi province haven’t infected CSFV.

Key words: CSFV; wild boars; RT-PCR; ELISA

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