RNAi导致基因沉默的机制和应用进展

›› 2007, Vol. 34 ›› Issue (3): 60-63.

RNAi导致基因沉默的机制和应用进展

向会耀,郝葆青，谭玉梅，秦天英，宋慧

西南民族大学生命科学院，成都 610041

收稿日期:1900-01-01 修回日期:1900-01-01 出版日期:2007-03-20 发布日期:2007-03-20

System and Development of RNAi Causing Gene Silence

XIANG Huiyao, HAO Baoqing, TAN Yumei, QIN Tianying, SONG Hui

Department of Animal Science and Technology Southwest University for Nationalities, Chengdu 610041,China

Received:1900-01-01 Revised:1900-01-01 Online:2007-03-20 Published:2007-03-20

摘要/Abstract

摘要： RNAi主要通过双链RNA(dsRNA)被核酸酶切割成21~23 nt的干涉性小的RNA,即siRNA,由siRNA介导识别并靶向切割同源性靶mRNA分子而实现的；RNAi需要多种蛋白质因子以及ATP参与,而且具有生物催化反应特征；RNAi具有高效性和高度特异性,作为一种关闭特定基因的新技术,为基因功能研究提供了一个快速、简便的方法,在后基因组时代应用前景广阔。

关键词: RNAi; 双链RNA; 基因沉默

Abstract: RNA interference(RNAi) is a post-transcriptional gene silencing induced by introduction of double-stranded RNA (dsRNA)。RNAi is achieved through incising by nucleic acid into small inference RNA of 21~23 nt by dsRNA, which is called siRNA, and is recognized and targeting incises the homologous mRNA molecular. RNAi needs various protein factors as well as ATP to participate in. It posesses the character of biocatalyzing, high efficiency and speciality. It has provided a fast and easy way for the study of the gene function as a new technology of shutting down fixed gene, which has an optimistic foreground in the post-genome time. 

Key words: RNAi; dsRNA; gene silence

中图分类号:

Q756

向会耀;郝葆青;谭玉梅;秦天英;宋慧. RNAi导致基因沉默的机制和应用进展[J]. , 2007, 34(3): 60-63.

XIANG Huiyao;HAO Baoqing;TAN Yumei;QIN Tianying;SONG Hui. System and Development of RNAi Causing Gene Silence [J]. China Animal Husbandry & Veterinary Medicine, 2007, 34(3): 60-63.

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