关键词: 辽宁绒山羊; 黎城大青羊; KAP6.2; KAP7; PCR-SSCP

Abstract: The polymerase chain reaction-single strand conformation polymorphism
（PCR-SSCP）was used to detected the polymorphism of keratin associated protein(KAP6.2, KAP7) in three goat breeds. The results show that the KAP7 didn't present polymorphism in all three breeds while the KAP6.2 shows three Genotypes: AA, AB and BB. The frequency of all pattern types analyzed by PCR-SSCP were Hardy We inberg equilibrium in Liaoning goat breed, however the same result didn't appear in Liaoning-Kelan crossed goat breed and LiCheng goat breed. The different genotype homozygote on KAP6.2 were cloned and sequenced. The results indicated that both AA and BB have nucleotide substitution , the AA genotype presents the following mutation: 107 bp(A-G)，196 bp(C-T)，199 bp(T-G)，252 bp(A-T)，265 bp(C-T)，291 bp(A-G)，292 bp(T-C)，345 bp(T-G)，and the BB genotype: 164 bp(T-C)，194 bp(T-C)，199 bp(T-G)，201 bp(C-G)，233 bp(A-G)，275 bp(A-G)，299 bp(A-G)，309 bp(G-C)，327 bp(A-G)，352 bp(G-T)。The sequence of amino acids was analyzed, the result showed that some of the mutations in AA and BB genotype caused amino acid change.

Key words: Liaoning cashmere goat; Licheng goat; KAP6.2; KAP7; PCR-SSCP