Cdc42基因对不同发育阶段无尾鸡骨骼的共表达调控

doi:10.16431/j.cnki.1671-7236.2021.11.001

中国畜牧兽医 ›› 2021, Vol. 48 ›› Issue (11): 3905-3917.doi: 10.16431/j.cnki.1671-7236.2021.11.001

Cdc42基因对不同发育阶段无尾鸡骨骼的共表达调控

齐旺梅¹, 胡斯乐², 徐斯日古楞¹, 哈登楚日亚¹, 白小英⁴, 张文广³

1. 内蒙古农业大学兽医学院, 呼和浩特 010018;
2. 内蒙古民族大学生命科学学院, 通辽 028000;
3. 内蒙古农业大学动物科学学院, 呼和浩特 010018;
4. 内蒙古通辽市科尔沁左翼中旗动物疫病预防控制中心, 通辽 028000

收稿日期:2021-04-21 出版日期:2021-11-20 发布日期:2021-11-01
通讯作者: 张文广 E-mail:actgnmbi@aliyun.com.cn
作者简介:齐旺梅(1974-),女,辽宁阜新人,博士,讲师,研究方向:鸡重要经济性状的遗传育种,E-mail:qiwangmei@imau.edu.cn
基金资助:
国家自然科学基金（31860640）

Co-expression Regulation of Cdc42 Gene in the Bone of Rumpless Chickens at Different Developmental Stages

QI Wangmei¹, HU Sile², XU Siriguleng¹, HA Dengchuriya¹, BAI Xiaoying⁴, ZHANG Wenguang³

1. College of Veterinary Medicine, Inner Mongolia Agricultural University, Hohhot 010018, China;
2. College of Life Science, Inner Mongolia University for Nationalities, Tongliao 028000, China;
3. College of Animal Sciences, Inner Mongolia Agricultural University, Hohhot 010018, China;
4. Animal Disease Prevention and Control Center of Keerqin Zuozhong Banner, Tongliao City, Inner Mongolia, Tongliao 028000, China

Received:2021-04-21 Online:2021-11-20 Published:2021-11-01

摘要/Abstract

摘要： 为阐明细胞分裂周期42（cell division cycle 42，Cdc42）基因在无尾鸡骨骼发育过程的潜在调控机制，本试验通过混池重测序与转录组测序方法，综合运用基因组学和骨骼转录组学来揭示Cdc42基因对无尾鸡尾部发育的遗传影响。结果显示，在无尾品种的强正向选择下，无尾鸡与有尾鸡之间高度分化的基因显著富集到神经发育、基础代谢与细胞骨架重排等生物类别；骨骼发育过程的差异表达基因数从胚胎第8、11和14天逐渐升高后下降，到第16天到达最低后又升高，到第21天达到最高；在基因组与转录组层面识别的公共候选基因为Cdc42；公共生物学过程富集到GTPases活性与细胞生长程度的调节，公共信号通路富集到紧密连接与黏着连接；差异表达基因构建的权重基因共表达网络发现，无尾鸡比有尾鸡的基因网络密度高6倍以上，平均连接度高8倍以上。无尾鸡共表达网络中，Cdc42基因与其他基因的相关性较弱。相比而言，在有尾鸡组，Cdc42基因处于网络的核心连接节点处，对于多基因的共同调节起到桥梁作用；此外，证明了Cdc42基因在多物种间具有保守性。本研究最终确定瓢鸡无尾性状的形成可能通过Cdc42及其共表达的基因共同调控紧密连接信号通路，联动影响Wnt信号通路，进而干扰生长板和尾骨的发育，是尾骨提前终止的重要影响环节，将为瓢鸡无尾表型的早期启动和遗传基础认识提供理论指导。

关键词: 无尾鸡; Cdc42基因; 骨骼发育; 基因组选择; 共表达基因

Abstract: To elucidate the potential regulatory mechanism of cell division cycle 42 (Cdc42) gene in the skeletal development of rumpless chicken, in this study, the hereditation of Cdc42 gene on tail development of rumpless chicken was revealed by genome pool resequencing and RNA sequencing, combined with genomics and bone development transcriptomics. Under the strong positive selection of the rumpless varieties, highly differentiated genes were significantly enriched in biological process such as neural development, basal metabolism and cytoskeleton rearrangement between rumpless and tailed chickens. The number of differentially expressed genes during bone development increased gradually next to decreased from the 8th, 11th and 14th day of embryo, on the 16th day it reached the lowest level and then rose again and reached the peak on the 21st day of embryo. The common candidate Cdc42 gene was identified at the genomics and transcriptomics levels. The common biological processes enriched in regulation of GTPase activity and the regulation of extent of cell growth. Common signaling pathways include tight junctions and adherens junctions. The weighted gene co-expression network analysis by differentially expressed genes showed that the density of gene network was more than 6 times higher and the average connectivity was more than 8 times higher in rumpless chicken than that of tailed chicken. The correlation between Cdc42 gene and other genes was low in the co-expression network of rumpless chicken. In contrast, Cdc42 gene was at the core of the network and acted as a bridge for the co-regulation of multiple genes in tailed chicken. In addition, Cdc42 gene was proved to be conserved among multiple species. In this study, it was finally determined that the formation of the rumpless trait in Piao chicken might co-regulate the tight junction signaling pathway through Cdc42 and its co-expressed genes, and interact with the Wnt signaling pathway, thus interfering with the development of growth plate and coccyx, and ultimately affecting the important link of premature termination of coccyx. These results would be provided theoretical guidance for the early initiation and genetic basis of rumpless phenotype.

Key words: rumpless chicken; Cdc42 gene; bone development; genome selection; co-expression gene

中图分类号:

S813

齐旺梅, 胡斯乐, 徐斯日古楞, 哈登楚日亚, 白小英, 张文广. Cdc42基因对不同发育阶段无尾鸡骨骼的共表达调控[J]. 中国畜牧兽医, 2021, 48(11): 3905-3917.

QI Wangmei, HU Sile, XU Siriguleng, HA Dengchuriya, BAI Xiaoying, ZHANG Wenguang. Co-expression Regulation of Cdc42 Gene in the Bone of Rumpless Chickens at Different Developmental Stages[J]. China Animal Husbandry and Veterinary Medicine, 2021, 48(11): 3905-3917.

参考文献

[1] TENIN G, WRIGHT D, FERJENTSIK Z, et al. The chick somitogenesis oscillator is arrested before all paraxial mesoderm is segmented into somites[J]. BMC Developmental Biology, 2010, 10(1):1-12.
[2] NOORAI R E, FREESE N H, WRIGHT L M, et al. Genome-wide association mapping and identification of candidate genes for the rumpless and ear-tufted traits of the Araucana Chicken[J]. PLoS One, 2012, 7(7):e40974.
[3] STOTT D, KISPERT A, HERRMANN B G.Rescue of the tail defect of Brachyury mice[J]. Gene Development, 1993, 7(2):197-203.
[4] WANG Q, PI J, PAN A, et al. A novel sex-linked mutant affecting tail formation in Hongshan chicken[J]. Scientific Reports, 2017, 7(1):10079.
[5] WOODS A, WANG G, DUPUIS H, et al. Rac1 signaling stimulates N-cadherin expression, mesenchymal condensation, and chondrogenesis[J]. The Journal of Biological Chemistry, 2007, 282(32):23500-23508.
[6] AIZAWA R, YAMADA A, SEKI T, et al. Cdc42 regulates cranial suture morphogenesis and ossification[J]. Biochemical and Biophysical Research Communications, 2019, 512(2):145-149.
[7] WANG G, BEIER F.Rac1/Cdc42 and RhoA GTPases antagonistically regulate chondrocyte proliferation, hypertrophy, and apoptosis[J]. Journal of Bone and Mineral Research, 2005, 20(6):1022-1031.
[8] SUZUKI W, YAMADA A, AIZAWA R, et al. Cdc42 is critical for cartilage development during endochondral ossification[J]. Endocrinology, 2015, 156(1):314-322.
[9] IKEHATA M, YAMADA A, FUJITA K, et al. Cooperation of Rho family proteins Rac1 and Cdc42 in cartilage development and calcified tissue formation[J]. Biochemical and Biophysical Research Communications, 2018, 500(3):525-529.
[10] RUBIN C J, ZODY M C, ERIKSSON J, et al. Whole-genome resequencing reveals loci under selection during chicken domestication[J]. Nature, 2010, 464(7288):587-591.
[11] GONGPAN P C, LIU L X, LI D L, et al. The investigation of genetic diversity of Piao chicken based on mitochondrial DNA D-loop region sequence[J]. Mitochondrial DNA, 2016, 27(4):211-223.
[12] LI M, TIAN S, YEUNG C K, et al. Whole-genome sequencing of Berkshire (European native pig) provides insights into its origin and domestication[J]. Scientific Reports, 2014, 4(4):4678.
[13] FREESE N H, LAM B A, STATON M, et al. A novel gain-of-function mutation of the proneural IRX1 and IRX2 genes disrupts axis elongation in the Araucana rumpless chicken[J]. PLoS One, 2014, 9(11):e112364.
[14] CAMBRAY N, WILSON V.Axial progenitors with extensive potency are localised to the mouse chordoneural hinge[J]. Development, 2002, 129(20):4855-4866.
[15] GONG H, WANG H, WANG Y, et al. Skin transcriptome reveals the dynamic changes in the Wnt pathway during integument morphogenesis of chick embryos[J]. PLoS One, 2018, 13(1):e0190933.
[16] 胡斯乐.有尾鸡与无尾鸡比较基因组学研究[D].呼和浩特:内蒙古农业大学, 2018. HU S L.Comparative genomic analysis of tailed chicken and rumples chicken[D].Hohhot:Inner Mongolia Agricultural University, 2018.(in Chinese)
[17] ZHANG W, XU Y, ZHANG L, et al. Synergistic effects of TGFβ2, WNT9a, and FGFR4 signals attenuate satellite cell differentiation during skeletal muscle development[J]. Aging Cell, 2018, 17(4):e12788.
[18] EI-SHARKAWY I, LIANG D, XU K.Transcriptome analysis of an apple (Malus×Domestica) yellow fruit somatic mutation identifies a gene network module highly associated with anthocyanin and epigenetic regulation[J]. Journal of Experimental Botany, 2015, 66(22):7359-7376.
[19] SCHLESINGER P H, BLAIR H C, BEER S D, et al. Cellular and extracellular matrix of bone, with principles of synthesis and dependency of mineral deposition on cell membrane transport[J]. American Journal of Physiology, 2020, 318(1):C111-C124.
[20] ALSHBOOL F Z, MOHAN S.Emerging multifunctional roles of claudin tight junction proteins in bone[J]. Endocrinology, 2014, 155(7):2363-2376.
[21] STAINS J P, CIVITELLI R.Cell-to-cell interactions in bone[J]. Biochemical and Biophysical Research Communication, 2005, 328(3):721-727.
[22] ARANA-CHAVEZ V E, SOARES A M, KATCHBURIAN E.Junctions between early developing osteoblasts of rat calvaria as revealed by freeze-fracture and ultrathin section electron microscopy[J]. Archives of Histology and Cytology, 1995, 58(3):285-292.
[23] TEUNISSEN M, RIEMERS F M, VAN LEENEN D, et al. Growth plate expression profiling:Large and small breed dogs provide new insights in endochondral bone formation[J]. Journal of Orthopaedic Research, 2018, 36(1):138-148.
[24] NELSON W J, NUSSE R.Convergence of Wnt, beta-catenin, and cadherin pathways[J]. Science, 303(5663):1483-1487.
[25] TUAN R S.Cellular signaling in developmental chondrogenesis:N-cadherin, Wnts, and BMP-2[J]. The Journal of Bone and Joint Surgery, 2003, 2:137-141.
[26] CHO S H, OH C D, KIM S J, et al. Retinoic acid inhibits chondrogenesis of mesenchymal cells by sustaining expression of N-cadherin and its associated proteins[J]. Journal of Cellular Biochemistry, 2003, 89(4):837-847.
[27] WANG Y, LI Y P, PAULSON C, et al. Wnt and the Wnt signaling pathway in bone development and disease[J]. Frontiers in Bioence, 2014, 19(3):379-407.
[28] LANDAUER W.Recessive rumplessness of fowl with kyphoscoliosis and supernumerary ribs[J]. Genetics, 1945, 30(5):403.
[29] HALL A, NOBES C D.Rho GTPases:Molecular switches that control the organization and dynamics of the actin cytoskeleton[J]. Philosophical Transactions of the Royal Society of London, 2000, 355(1399):965-970.
[30] ETIENNE-MANNEVILLE S, HALL A.Rho GTPases in cell biology[J]. Nature, 2002, 420(6916):629-635.
[31] WANG G, WOODS A, AGOSTON H, et al. Genetic ablation of Rac1 in cartilage results in chondrodysplasia[J]. Developmental Biology, 2007, 306(2):612-623.
[32] GAO J, MA S, YANG F, et al. MiR-193b exhibits mutual interaction with MYC, and suppresses growth and metastasis of osteosarcoma[J]. Oncology Reports, 2020, 44(1):139-155.
[33] WANG Y M, KHEDERZADEH S, LI S R, et al. Integrating genomic and transcriptomic data to reveal genetic mechanisms underlying Piao chicken rumpless trait[J]. Genomics Proteomics Bioinformatics, 2021, 21:S1672.
[34] CHEN F, MA L, PARRINI M C, et al. Cdc42 is required for PIP(2)-induced actin polymerization and early development but not for cell viability[J]. Current Biology, 2000, 10(13):758-765.
[35] 胡斯乐, 陶萨如拉, 王月星, 等.鸡无尾性状的分子机制研究进展[J]. 中国畜牧兽医, 2018, 45(1):154-161. HU S L, TAO S, WANG Y X, et al. Research progress on molecular mechanisms of rumples trait in chicken[J]. China Animal Husbandry & Veterinary Medicine, 2018, 45(1):154-161.(in Chinese)

Cdc42基因对不同发育阶段无尾鸡骨骼的共表达调控

Co-expression Regulation of Cdc42 Gene in the Bone of Rumpless Chickens at Different Developmental Stages

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