激素剂量、超排间隔及重复超排次数对和牛重复超排效果的影响

doi:10.16431/j.cnki.1671-7236.2021.09.031

摘要/Abstract

摘要： 试验旨在探讨激素剂量、超排间隔及重复超排次数对和牛重复超排效果的影响，为建立稳定、高效的和牛供体重复超排方案提供依据。本试验共选取63头15~18月龄的和牛供体母牛进行超排处理，其中17头和牛供体随机分成2组，分别以200、200、200 mg的FSH注射总剂量和200、220、240 mg的FSH注射总剂量进行3次重复超排，每次超排间隔30 d，研究激素剂量对和牛重复超排的影响；24头和牛供体随机分成3组，每组分别以60、45、30 d的超排间隔，每次200 mg的FSH注射总剂量进行3次重复超排，研究超排间隔对和牛重复超排的影响；22头和牛供体采用每次200 mg的FSH注射总剂量进行连续7次重复超排，每次超排间隔30 d，研究重复超排次数对和牛重复超排的影响。结果显示，采用FSH注射总剂量为200、220、240 mg获得的头均胚胎总数和头均可用胚胎数显著高于每次均为200 mg的FSH注射总剂量（P<0.05）；与超排间隔60 d组相比，超排间隔45 d组获得的头均可用胚胎数显著降低（P<0.05），而超排间隔30 d组获得的头均可用胚胎数也降低，但差异不显著（P>0.05）；重复超排在6次以内，获得的头均可用胚胎数无显著差异（P>0.05）。结果表明，注射FSH总剂量为200、220、240 mg连续超排3次的超排效果优于注射FSH总剂量为200、200、200 mg组；超排间隔越长，越有利于母牛生殖道的恢复，获得的可用胚胎数越多，但超排间隔30 d，也可获得良好的超排效果；高强度重复超排以30 d为超排间隔，超排5~6次为宜。

关键词: 和牛; 重复超排; 激素剂量; 超排间隔; 可用胚

Abstract: The aim of this study was to investigate the effects of hormone dose, interval of superovulation and times of repeated superovulation on superovulation in Wagyu cattle, in order to provide evidence for establishing a stable and efficient repeated superovulation protocol. 63 Wagyu cattles aged 15-18-month-old were selected as superovulation donors. Among them, 17 donors were randomly divided into two groups, injected with a total dose of 200, 200, 200 mg FSH and a total dose of 200, 220, 240 mg FSH, respectively. Repeated superovulation was performed for 3 times with an interval of 30 days, to study the effect of hormone dosages on superovulation. 24 donors were randomly divided into three groups, each group was injected 200 mg dose of FSH for 3 consecutive times superovulation for 60, 45 and 30 days treated intervals, respectively, to study the effect of interval time of hormone injection on superovulation. 22 donors were injected with 200 mg FSH each time for 7 consecutive repetitions with an interval of 30 days, to study the effect of repetition times on superovulation. The results showed that the total number of embryos and the total number of available embryos obtained by the donors received 200, 220 and 240 mg doses of FSH were significantly higher than those obtained by the donors subjected to 200 mg dose of FSH treatment (P<0.05). By comparison with the superovulation interval of 60 days group, the number of available embryos produced at an interval of 45 days was significantly decreased (P<0.05), and the number of available embryos obtained by the donors in the 30 days interval group was also decreased, but there was no significant difference (P>0.05). There was no significant difference in the number of available embryos when repeated superovulation was less than 6 times (P>0.05). In summary, the effect of 200, 220 and 240 mg doses of FSH on repeated superovulation was better than that of 200, 200 and 200 mg dosage group. Longer superovulation interval was benefit to the recovery of reproductive tract and the available of available embryos, but the repetitious superovulation at 30 days interval could also get satisfactory results. The suitable number of superovulation times was 5 to 6 when the interval was 30 days for high intensive repeated superovulation.

Key words: Wagyu cattle; repeated superovulation; hormone dosage; superovulation interval; available embryos

中图分类号:

S823

王林, 庞云渭, 郝海生, 宋金辉, 赵善江, 杜卫华, 赵学明, 邹惠影, 朱化彬. 激素剂量、超排间隔及重复超排次数对和牛重复超排效果的影响[J]. 中国畜牧兽医, 2021, 48(9): 3394-3402.

WANG Lin, PANG Yunwei, HAO Haisheng, SONG Jinhui, ZHAO Shanjiang, DU Weihua, ZHAO Xueming, ZOU Huiying, ZHU Huabin. Effects of Hormone Dosage,Superovulation Interval and Repeated Times on Repeated Superovulation in Wagyu Cattle[J]. China Animal Husbandry and Veterinary Medicine, 2021, 48(9): 3394-3402.

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