环境雌激素灭多威对牛卵母细胞体外培养质量的影响

doi:10.16431/j.cnki.1671-7236.2020.09.022

中国畜牧兽医 ›› 2020, Vol. 47 ›› Issue (9): 2884-2891.doi: 10.16431/j.cnki.1671-7236.2020.09.022

环境雌激素灭多威对牛卵母细胞体外培养质量的影响

何道宏^1,2,3, 韩国波^1,2,3, 孙敬宇^1,2,3, 崔宇新^1,2,3, 殷颖^1,2,3, 张笑梦^1,2,3, 鄂志强^1,2,3, 高青山^1,2,3

1. 延边大学农学院, 延吉 133002;
2. 东北寒区肉牛科技创新教育部工程研究中心, 延吉 133002;
3. 延边黄牛种质资源保护工程研究中心, 延吉 133002

收稿日期:2020-05-26 出版日期:2020-09-20 发布日期:2020-09-16
通讯作者: 高青山 E-mail:qsgao@ybu.edu.cn
作者简介:何道宏(1994-),男,安徽六安人,硕士生,研究方向:动物遗传育种与繁殖,E-mail:daohonghe@126.com
基金资助:
吉林省发改委延边黄牛种质资源保护与开发利用项目（2020C037-4）；高等学校学科创新引智计划项目（D20034）

Effect of Environmental Estrogen Methomyl on the Quality of in vitro Culture Bovine Oocytes

HE Daohong^1,2,3, HAN Guobo^1,2,3, SUN Jingyu^1,2,3, CUI Yuxin^1,2,3, YIN Ying^1,2,3, ZHANG Xiaomeng^1,2,3, E Zhiqiang^1,2,3, GAO Qingshan^1,2,3

1. Agricultural College of Yanbian University, Yanji 133002, China;
2. Engineering Research Center of North-East Cold Region Beef Cattle Science & Technology Innovation, Ministry of Education, Yanji 133002, China;
3. Jilin Engineering Research Center of Yanbian Yellow Cattle Resources Reservation, Yanji 133002, China

Received:2020-05-26 Online:2020-09-20 Published:2020-09-16

摘要/Abstract

摘要： 试验旨在研究体外培养液中添加灭多威（methomyl，MET）对牛卵母细胞质量的影响。通过免疫荧光染色和实时荧光定量PCR等方法检测牛卵母细胞的卵丘细胞扩展程度、第一极体排出率、活性氧（reactive oxygen species，ROS）水平、谷胱甘肽（glutathione，GSH）水平、线粒体膜电位（ΔΨm）以及凋亡相关基因的表达进行评价。结果显示，不同浓度MET不影响卵丘细胞的扩展程度，但200 μmol/L MET处理组卵母细胞的第一极体排出率显著低于对照组（P<0.05）。与对照组相比，200 μmol/L MET处理组的卵母细胞ROS水平显著升高，GSH水平、线粒体膜电位（ΔΨm）均显著降低（P<0.05）。此外，本研究还发现，200 μmol/L MET处理组卵母细胞中促凋亡基因Caspase-3和Bax的mRNA转录水平显著升高，抗凋亡基因Bcl-xl的mRNA转录水平显著降低（P<0.05）。上述结果表明，MET可以通过增加氧化应激和细胞凋亡来降低体外培养牛卵母细胞的质量。

关键词: 牛; 灭多威; 氧化应激; 线粒体; 卵母细胞

Abstract: The aim of this study was to evaluate the effects of methotyl (MET) on the quality of in vitro cultured bovine oocytes.The quality of bovine oocytes was evaluated using the first polar body excretion rate,reactive oxygen species (ROS),glutathione (GSH) level,mitochondrial membrane potential (ΔΨm) and the expression of apoptosis-related gene.The results demonstrated that different concentration of MET had no effects on cumulus cells expansion,but the first polar body excretion rate was significantly lower than that of control group after administration of 200 μmol/L MET (P<0.05).The data also showed that 200 μmol/L MET could significantly increase ROS and decrease GSH and mitochondrial membrane potential (P<0.05).In addition,the mRNA transcription levels of Caspase-3 and Bax,which were pro-apoptotic genes,were significantly increased and the anti-apoptotic gene Bcl-xl was significantly reduced after MET administration (P<0.05).The results indicated that MET could increase oxidative stress and apoptosis which led to the reduction of bovine oocytes quality.

Key words: bovine; methomyl; oxidative stress; mitochondria; oocyte

中图分类号:

S823.8⁺1

何道宏, 韩国波, 孙敬宇, 崔宇新, 殷颖, 张笑梦, 鄂志强, 高青山. 环境雌激素灭多威对牛卵母细胞体外培养质量的影响[J]. 中国畜牧兽医, 2020, 47(9): 2884-2891.

HE Daohong, HAN Guobo, SUN Jingyu, CUI Yuxin, YIN Ying, ZHANG Xiaomeng, E Zhiqiang, GAO Qingshan. Effect of Environmental Estrogen Methomyl on the Quality of in vitro Culture Bovine Oocytes[J]. China Animal Husbandry and Veterinary Medicine, 2020, 47(9): 2884-2891.

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环境雌激素灭多威对牛卵母细胞体外培养质量的影响

Effect of Environmental Estrogen Methomyl on the Quality of in vitro Culture Bovine Oocytes

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