鸭疫里默氏菌对鸭盲肠组织形态结构和TLR4信号通路相关基因表达的影响

doi:10.16431/j.cnki.1671-7236.2020.06.008

中国畜牧兽医 ›› 2020, Vol. 47 ›› Issue (6): 1702-1708.doi: 10.16431/j.cnki.1671-7236.2020.06.008

鸭疫里默氏菌对鸭盲肠组织形态结构和TLR4信号通路相关基因表达的影响

陶志云¹, 朱春红¹, 施祖灏², 徐文娟¹, 章双杰¹, 宋卫涛¹, 刘宏祥¹, 李慧芳¹

1. 江苏省家禽科学研究所, 扬州 225125;
2. 谱尼测试集团江苏有限公司, 苏州 215000

收稿日期:2019-11-08 出版日期:2020-06-20 发布日期:2020-06-20
通讯作者: 李慧芳 E-mail:lhfxf_002@aliyun.com.cn
作者简介:陶志云(1979-),女,安徽滁州人,博士,副研究员,研究方向:家禽免疫及遗传育种,E-mail:zhiyun2@126.com
基金资助:
江苏省第五期"333高层次人才培养工程"（BRA2018080）；扬州市现代农业项目（YZ2018046）；江苏省公益类科研院所自主科研项目（BM2018026）；江苏省现代农业（水禽）产业技术体系（JATS[2019]347）

Effect of Riemerella anatipestifer on the Morphological Structure and TLR4 Signal Pathway Related Gene Expression of Cecum in Ducks

TAO Zhiyun¹, ZHU Chunhong¹, SHI Zuhao², XU Wenjuan¹, ZHANG Shuangjie¹, SONG Weitao¹, LIU Hongxiang¹, LI Huifang¹

1. Jiangsu Institute of Poultry Science, Yangzhou 225125, China;
2. Pony Testing Group Jiangsu Co., Ltd., Suzhou 215000, China

Received:2019-11-08 Online:2020-06-20 Published:2020-06-20

摘要/Abstract

摘要： 为探讨鸭疫里默氏菌的可能致病机制，本试验采用显微镜观察鸭疫里默氏菌感染后鸭盲肠组织的形态结构变化，并对肠黏膜厚度、肠绒毛高度及隐窝深度进行测量和统计，采用实时荧光定量PCR法检测鸭疫里默氏菌感染后盲肠组织中TLR4信号通路相关基因的表达变化。病理学观察结果显示，鸭疫里默氏菌感染后肠道结构有明显的损伤，表现为肠绒毛脱落、淤血、出血、淋巴细胞浸润和淋巴细胞增生；统计结果表明，2 d时，鸭疫里默氏菌感染组的肠黏膜厚度（383.58 μm）显著低于对照组（643.39 μm）（P<0.05）；2和5 d时，感染组的肠绒毛高度（173.04和168.68 μm）均显著低于对照组（355.79和276.54 μm）（P<0.05）；9 d时，鸭疫里默氏菌感染组的绒毛高度/隐窝深度的比值（3.42）显著低于对照组（5.34）（P<0.05）。实时荧光定量PCR结果显示，鸭疫里默氏菌感染后2和5 d，TLR4信号通路相关基因TLR4、MD2、MyD88、TRAF6、NF-κB、IL-4和IL-8 mRNA的表达量上调。说明鸭疫里默氏菌感染可导致盲肠的肠黏膜组织物理损伤和炎性病变，且TLR4信号通路参与了该炎性反应过程。

关键词: 鸭疫里默氏菌; 盲肠; 形态结构; TLR4信号通路

Abstract: In order to investigate the possible pathogenic mechanism of Riemerella anatipestifer,the morphological structure was observed using microscope,the thickness of intestinal mucosa,the height of intestinal villi and the depth of crypt were measured and counted,and the expression of TLR4 signal pathway related genes in the cecum of ducks after infected with Riemerella anatipestifer was detected by Real-time quantitative PCR.Pathological observation results showed that the intestinal structure was damaged after Riemerella anatipestifer infection,which was manifested as intestinal villus shedding,congestion,bleeding and lymphocyte infiltration and lymphocyte proliferation.The statistical results showed that the thickness of intestinal mucosa in infection group (383.58 μm) was significantly lower than that in control group (643.39 μm) at 2 d (P<0.05).The height of intestinal villi in infection group (173.04 and 168.68 μm) were significantly lower than that in control group (355.79 and 276.54 μm) at 2 and 5 day (P<0.05),respectively.The ratio of height of intestinal villi to crypt depth in infection group (3.42) was significantly lower than that of control group (5.34) at 9 d (P<0.05).Real-time quantitative PCR results showed that the expression of TLR4 signal pathway related genes of TLR4,MD2,MyD88,TRAF6,NF-κB,IL-4 and IL-8 mRNA was up-regulated at 2 and 5 d after infection.These results suggested that the infection of Riemerella anatipestifer could lead to the physical damage and inflammatory lesions of the cecum,and TLR4 signal pathway was involved in the inflammatory response process.

Key words: Riemerella anatipestifer; cecum; morphological structure; TLR4 signal pathway

中图分类号:

Q786

陶志云, 朱春红, 施祖灏, 徐文娟, 章双杰, 宋卫涛, 刘宏祥, 李慧芳. 鸭疫里默氏菌对鸭盲肠组织形态结构和TLR4信号通路相关基因表达的影响[J]. 中国畜牧兽医, 2020, 47(6): 1702-1708.

TAO Zhiyun, ZHU Chunhong, SHI Zuhao, XU Wenjuan, ZHANG Shuangjie, SONG Weitao, LIU Hongxiang, LI Huifang. Effect of Riemerella anatipestifer on the Morphological Structure and TLR4 Signal Pathway Related Gene Expression of Cecum in Ducks[J]. China Animal Husbandry and Veterinary Medicine, 2020, 47(6): 1702-1708.

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鸭疫里默氏菌对鸭盲肠组织形态结构和TLR4信号通路相关基因表达的影响

Effect of Riemerella anatipestifer on the Morphological Structure and TLR4 Signal Pathway Related Gene Expression of Cecum in Ducks

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