多因素法构建肉鸡腹水综合征实验模型及其对肺组织血管内皮舒缩活性因子的影响

doi:10.16431/j.cnki.1671-7236.2020.05.007

摘要/Abstract

摘要： 试验旨在研究多因素法构建肉鸡腹水综合征（AS）实验模型及其对肺组织血管内皮舒缩活性因子的影响。将158羽健康罗斯肉鸡随机分为空白组、低温模型组和多因素模型组，于25、35、45日龄随机取样，观察临床症状、剖检变化及肺组织病理变化，测定体重、发病率、腹水心脏指数（AHI），检测肺组织一氧化氮（NO）、内皮素-1（ET-1）、一氧化氮合酶（NOS）变化，并以实时荧光定量PCR法检测肺组织内皮素A型受体（ETAR）及血管内皮生长因子（VEGF）mRNA表达量。结果显示，各日龄低温模型组和多因素模型组肉鸡AHI均极显著高于空白组（P<0.01），各日龄多因素模型组肉鸡AHI均显著高于低温模型组（P<0.05）；35、45日龄低温模型组和各日龄多因素模型组肉鸡体重显著或极显著低于空白组（P<0.05，P<0.01）；35、45日龄低温模型组和各日龄多因素模型组AS发病率均极显著高于空白组（P<0.01），25日龄多因素模型组AS发病率极显著高于低温模型组（P<0.01）；35日龄AS肉鸡肺组织结构损伤，内皮细胞脱落、平滑肌排列紊乱；与空白组相比，35、45日龄低温模型组和多因素模型组肺组织NO与ET-1含量、iNOS活性、ETAR及VEGF mRNA表达量均显著或极显著升高（P<0.05，P<0.01），肺组织NO/ET-1、cNOS与TNOS活性均显著或极显著降低（P<0.05，P<0.01）。表明低温、高钠、高能饲料多因素法能够成功构建AS实验模型，多因素造模法较低温造模法更能反映疾病因素的多样性；肺组织血管内皮舒缩活性因子表达紊乱参与了AS的发生发展。

关键词: 多因素法; 腹水综合征(AS); 肺组织; 血管内皮舒缩活性因子

Abstract: This experiment was aimed to study the experimental model of broiler ascites syndrome (AS) by multi-factor method and its effect on diastolic-systolic factors of vascular endothelium in lung tissue.A total of 158 healthy Ross broilers were randomly divided into normal group,low temperature model group and multi-factor model group.At 25,35 and 45 days of age,the clinical symptoms,post-mortem change and the pathological change of lung tissue were observed,the body weight,the incidence of AS and ascites heart index (AHI) were measured,and the change of NO,ET-1 and NOS were detected,and the expression of ETAR and VEGF genes in lung tissue were detected by Real-time quantitative PCR.The results showed that the AHI of broilers at each day-age in the low temperature and the multi-factor model groups was extremely significantly higher than that in the normal group (P<0.01),the AHI of broilers at each day-age in the multi-factor model group was significantly higher than that in the low temperature model group (P<0.05).The body weight of broilers at 35 and 45 days of age in the low temperature model group and each day-age in the multi-factor model group were significantly or extremely significantly lower than that in the normal group (P<0.05,P<0.01).The incidence of AS at 35 and 45 days of age in the low temperature model group and each day-age in the multi-factor model group were extremely significantly higher than that in the normal group (P<0.01).At 25 days of age,the incidence of AS in the multi-factor model group was extremely significantly higher than that in the low temperature model group (P<0.01).At 35 days of age,lung tissue of broilers with AS structure was damaged,endothelial cells were shed,and smooth muscle arrangement was disturbed.Compared with the normal group,the contents of NO and ET-1,iNOS activity,ETAR and VEGF mRNA expression in lung tissue at 35 and 45 days of age in the low temperature and the multi-factor model groups were significantly or extremely significantly increased (P<0.05,P<0.01),the ratio of NO to ET-1 and the activity of cNOS and TNOS were significantly or extremely significantly reduced (P<0.05,P<0.01).Experimental ascites syndrome model in broiler could be successfully established induced by multi-factor method with low temperature,high sodiu and high energy feed.The multi-factor model method could reflect the diversity of disease factors better than the low temperature model method.The expression disorder of diastolic-systolic factors of vascular endothelium in lung tissue was involved in the occurrence of broiler AS.

Key words: multi-factor method; ascites syndrome (AS); lung tissue; diastolic-systolic factors of vascular endothelium

中图分类号:

S856.5

郭宇荣, 崔亮, 苏晓菲, 王卓, 朱昱波, 李佩岐, 张剑, 孙涵燕, 员世宇, 段智变. 多因素法构建肉鸡腹水综合征实验模型及其对肺组织血管内皮舒缩活性因子的影响[J]. 中国畜牧兽医, 2020, 47(5): 1342-1351.

GUO Yurong, CUI Liang, SU Xiaofei, WANG Zhuo, ZHU Yubo, LI Peiqi, ZHANG Jian, SUN Hanyan, YUN Shiyu, DUAN Zhibian. Establishment of Ascites Syndrome Model in Broiler Induced by Multi-factor and Its Effect on Diastolic-systolic Factors of Vascular Endothelium in Lung Tissue[J]. China Animal Husbandry and Veterinary Medicine, 2020, 47(5): 1342-1351.

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