《中国畜牧兽医》 ›› 2016, Vol. 43 ›› Issue (8): 2164-2169.doi: 10.16431/j.cnki.1671-7236.2016.08.032

• 疾病防治 • 上一篇    下一篇

酵母诱导表达鸡IFN-α抗IBDV效果及其对信号分子PI3K和NF-κB的影响

韩海锋1, 李银聚1, 程相朝1, 张春杰1, 余祖华1, 杨惠1, 杨荣恩2, 付威2   

  1. 1. 河南科技大学动物疫病与公共安全重点实验室, 洛阳 471003;
    2. 河南天纳图生物科技有限公司, 漯河 462600
  • 收稿日期:2015-12-28 出版日期:2016-08-20 发布日期:2016-08-23
  • 通讯作者: 李银聚 E-mail:lyj6511@126.com
  • 作者简介:韩海锋(1989-),男,河南洛阳人,硕士生,研究方向:病毒分子生物学,E-mail:haifeng_han@139.com
  • 基金资助:

    河南省科技攻关项目(132102110117)

Study on the Anti-IBDV Activity of Chicken IFN-α and Its Effects on Signaling Molecule PI3K and NF-κB

HAN Hai-feng1, LI Yin-ju1, CHENG Xiang-chao1, ZHANG Chun-jie1, YU Zu-hua1, YANG Hui1, YANG Rong-en2, FU Wei2   

  1. 1. Key Laboratory of Animal Disease and Public Security, Henan University of Science and Technology, Luoyang 471003, China;
    2. Henan Tian Na Tu Biotechnology Co., Ltd., Luohe 462600, China
  • Received:2015-12-28 Online:2016-08-20 Published:2016-08-23

摘要:

试验旨在研究酵母表达鸡IFN-α抗传染性法氏囊病病毒(IBDV)的效果及对其淋巴细胞信号分子PI3K和NF-κB p65的影响。将40只10日龄非免疫雏鸡随机分为IFN-α组和空白对照组,持续注射给药3 d后,于第3次给药后第1天开始每天心脏采血,持续3 d,并于最后一次采血后对雏鸡进行攻毒,在攻毒后开始每天心脏采血,连续3 d。通过MTT法检测淋巴增殖活性情况,ELISA测定不同时间段淋巴细胞中PI3K的水平、细胞中总NF-κB p65、细胞核中NF-κB p65蛋白表达含量及IBDV抗原量。结果显示,与对照组相比,攻毒前,IFN-α对淋巴增殖活性、细胞中PI3K和NF-κB p65的表达具有极显著地促进作用(P<0.01);与攻毒前相比,攻毒后第1和2天IFN-α组淋巴增殖活性、细胞中PI3K和NF-κB p65的表达极显著增加(P<0.01),IFN-α对IBDV-Ag具有极显著地抑制效果(P<0.01)。这些结果表明,IFN-α可通过增加PI3K激活NF-κB信号通路以增强机体免疫反应,并对IBDV-Ag有显著的抑制作用,本试验为IFN-α免疫增强和抗病毒作用的研究提供科学依据。

关键词: IFN-α PI3K; 核转录因子; NF-κ B p65; 传染性法氏囊病

Abstract:

To investigate the anti-IBDV activity of IFN-α and its effects on chicken lymphocyte signaling molecule PI3K and NF-κB activity,forty 10-day-old non-immune chickens were randomly separated into two groups:Blank control group and IFN-α group.The chicken of two groups were inoculated with IFN-α and saline via intramscular injection for three days,respectively.Then they were inoculated with IBDV.From 13 to 18-day-old,the lymphocytes were isolated from peripheral blood of chicken.The lymphocyte proliferation of chicken were detected by the MTT method,the PI3K and NF-κB p65 concentration in lymphocyte and the IBDV-Ag of serum were detected at different time with ELISA.The results showed that compared with the blank control group,IFN-α could stimulate chicken lymphocyte proliferation and the PI3K and NF-κB p65 concentration in chicken lymphocyte were extremely significantly increased before infection (P<0.01).The lymphocyte proliferation and the expression of PI3K and NF-κB p65 in cell after IBDV infection were extremely significantly higher than before (P<0.01).IFN-α could extremely significantly inhabit the proliferation of IBDV-Ag (P<0.01).IFN-α could significantly promote lymphocyte transformation by increasing PI3K concentration and the biological activity of lymphocyte transformation associated with the PI3K and NF-κB p65 expression,activation and nucleo-cytoplasmic transport.It also could reduce IBDV-Ag proliferation in chickens.The study built foundation for IFN-α protein immuno enhancement and anti-viral activity.

Key words: IFN-α; PI3K; nuclear factor; NF-κB p65; IBD

中图分类号: