小鼠Oct-1基因CDS区克隆与生物信息学分析

doi:10.16431/j.cnki.1671-7236.2016.08.005

《中国畜牧兽医》 ›› 2016, Vol. 43 ›› Issue (8): 1960-1966.doi: 10.16431/j.cnki.1671-7236.2016.08.005

小鼠Oct-1基因CDS区克隆与生物信息学分析

杨玉静¹, 聂瑞强¹, 谢建山^1,2, 范瑞文¹, 董常生¹

1. 山西农业大学动物科技学院, 太谷 030801;
2. 山西医科大学基础医学院, 太原 030001

修回日期:2016-05-03 出版日期:2016-08-20 发布日期:2016-08-23
通讯作者: 董常生 E-mail:cs_dong@sxau.edu.cn
作者简介:杨玉静(1991-),女,山西长治人,硕士生,研究方向:动物组织学与胚胎工程,E-mail:15110679040@163.com
基金资助:
国家高科技研究发展计划（2013AA102506）；公益性行业（农业）科研专项（201303119）；山西农业大学创新团队

Cloning and Bioinformatics Analysis on CDS of Oct-1 Gene in Mouse

YANG Yu-jing¹, NIE Rui-qiang¹, XIE Jian-shan^1,2, FAN Rui-wen¹, DONG Chang-sheng¹

1. College of Animal Science and Veterinary Medicine, Shanxi Agricultural University, Taigu 030801, China;
2. School of Basic Medical Sciences, Shanxi Medical University, Taiyuan 030001, China

Revised:2016-05-03 Online:2016-08-20 Published:2016-08-23

摘要/Abstract

摘要：

为研究小鼠Oct-1基因的生物学特性，试验扩增小鼠黑色素细胞中Oct-1基因CDS区序列，并运用生物信息学软件分析小鼠Oct-1基因序列的特性、编码蛋白理化性质、亚细胞定位、靶基因及保守性结构域等，从而预测其是否调控黑色素的生成。结果表明，小鼠Oct-1基因大小为2 313 bp，编码蛋白质的分子式C₃₄₂₅H₅₆₀₆N₉₇₆O₁₁₆₃S₁₅，是一个不稳定可溶性蛋白质，二级结构以无规卷曲和α-螺旋为主，亚细胞定位主要分布在细胞核，推测其可能在能量代谢和辅因子的生物合成过程中发挥信号转导和转录因子调控的作用；Oct-1基因在小鼠黑色素细胞中表达，其编码的蛋白含有一个保守的POU结构域和一个保守的同源域，参与调控13个黑色素形成相关基因转录表达，在黑色素生成关键基因MITF、TYR、TYRP1、TYRP2的启动子上存在Oct-1转录因子的作用位点，Oct-1蛋白质还可能与Brf2、Pou2af1、Tbp等蛋白相互作用调控毛色基因表达，故可以推测转录因子Oct-1在黑素细胞的黑色素生成中发挥着重要的作用，为研究毛色形成机制提理论依据。

关键词: Oct-1基因; 黑色素细胞; 基因克隆; 生物信息学

Abstract:

In order to explore the biological characteristics of Oct-1 gene in mouse,CDS region of Oct-1 gene in mouse was cloned.And the sequence,physicochemical property,subcellular localization,target genes and conservative structure domain of Oct-1 gene were analyzed by bioinformatics method to predict whether the Oct-1 gene could regulate the production of melanin.The results showed that the length of Oct-1 gene in mouse was 2 313 bp.The formula of Oct-1 protein was C₃₄₂₅H₅₆₀₆N₉₇₆O₁₁₆₃S₁₅,which was an unstable and soluble protein.The secondary structure of Oct-1 mainly contained random coil and α-helices.Subcellular localization of Oct-1 was mainly in the nucleus,which Oct-1 might play a role of signal transducer or transcription regulation in the energy metabolism and cofactor biosynthesis.The Oct-1 gene regulated the expression of 13 genes associated with the melanin formation of mouse.The protein was encoded by Oct-1 gene including a homeo-domain and a POU domain.There were binding sites of Oct-1 on the promoters of MITF,TYR,TYRP1 and TYRP2. Oct-1 might interact with Brf2,Pou2af1 and Tbp to regulate the colour gene expression.We inferred that the transcription factor Oct-1 played an important role in melanogenesis and these results provided a theoretical basis for studying the coat colour formation mechanism.

Key words: Oct-1 gene; melanocytes; gene cloning; bioinformatics

中图分类号:

Q785

杨玉静, 聂瑞强, 谢建山, 范瑞文, 董常生. 小鼠Oct-1基因CDS区克隆与生物信息学分析[J]. 《中国畜牧兽医》, 2016, 43(8): 1960-1966.

YANG Yu-jing, NIE Rui-qiang, XIE Jian-shan, FAN Rui-wen, DONG Chang-sheng. Cloning and Bioinformatics Analysis on CDS of Oct-1 Gene in Mouse[J]. , 2016, 43(8): 1960-1966.

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小鼠Oct-1基因CDS区克隆与生物信息学分析

Cloning and Bioinformatics Analysis on CDS of Oct-1 Gene in Mouse

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