紫菀乙醇提取物对豚鼠离体气管平滑肌收缩功能的影响

doi:10.16431/j.cnki.1671-7236.2016.06.026

›› 2016, Vol. 43 ›› Issue (6): 1572-1578.doi: 10.16431/j.cnki.1671-7236.2016.06.026

紫菀乙醇提取物对豚鼠离体气管平滑肌收缩功能的影响

彭文静¹, 辛蕊华^1,2,3,4, 刘艳¹, 罗永江^1,2,3,4, 王贵波^1,2,3,4, 罗超应^1,2,3,4, 谢家声^1,2,3,4, 李锦宇^1,2,3,4, 郑继方^1,2,3,4

1. 中国农业科学院兰州畜牧与兽药研究所, 兰州 730050;
2. 甘肃省新兽药工程重点实验室, 兰州 730050;
3. 甘肃省中兽药工程技术研究中心, 兰州 730050;
4. 农业部兽用药物创制重点实验室, 兰州 730050

收稿日期:2015-12-04 出版日期:2016-06-20 发布日期:2016-07-11
通讯作者: 郑继方 E-mail:zhengjifang100@126.com
作者简介:彭文静(1989-),女,湖南衡阳人,硕士生,研究方向:中兽医药学,E-mail:pengwenjingzy@163.com
基金资助:
甘肃省科技支撑计划(1304NKCA155);公益性行业(农业)科研专项(201303040-18)

Effects of Alcohol Extract of Aster tataricus L.f.on the Contraction of Guinea Pig Tracheal Smooth Muscle in vitro

PENG Wen-jing¹, XIN Rui-hua^1,2,3,4, LIU Yan¹, LUO Yong-jiang^1,2,3,4, WANG Gui-bo^1,2,3,4, LUO Chao-ying^1,2,3,4, XIE Jia-sheng^1,2,3,4, LI Jin-yu^1,2,3,4, ZHENG Ji-fang^1,2,3,4

1. Lanzhou Institute of Husbandry and Pharmaceutical Sciences of CAAS, Lanzhou 730050, China;
2. Key Laboratory of New Animal Drug Project of Gansu Province, Lanzhou 730050, China;
3. Engineering & Technology Research Center of Traditional Chinese Veterinary Medicine of Gansu Province, Lanzhou 730050, China;
4. Key Laboratory of Veterinary Pharmaceutics Development, Ministry of Agriculture, Lanzhou 730050, China

Received:2015-12-04 Online:2016-06-20 Published:2016-07-11

摘要/Abstract

摘要： 本试验旨在研究紫菀乙醇提物对豚鼠离体气管平滑肌收缩功能的影响。采用离体气管恒温灌流的方法,将豚鼠气管制成气管螺旋条,通过 BL-420F 生物机能实验系统测定其张力的变化,观察紫菀乙醇提物对离体气管平滑肌静息状态下的舒张作用,以及在氯化乙酰胆碱(Ach)、磷酸组胺(His)、CaCl₂条件下和无钙下Ach诱导细胞内钙释放和外钙内流所致两种收缩条件下对离体气管平滑肌张力变化的影响。试验结果显示,低浓度紫菀乙醇提取物(0.002~0.008 g/mL)对静息状态下豚鼠离体气管平滑肌具有一定的收缩作用,高浓度(0.008~0.196 g/mL)时具有舒张作用;低、中、高3个剂量组均可抑制Ach、His和CaCl₂引起的气管平滑肌收缩强度,使各致痉剂的量效曲线非平行右移并降低最大效应。紫菀乙醇提取物对His的抑制作用强度最大,其次为Ach,最后是CaCl₂,且均呈剂量依赖性。以上结果表明紫菀乙醇提物具有舒张气管平滑肌的作用,其机制可能与抑制豚鼠气管平滑肌M受体、H₁受体和阻断Ca²⁺通道从而抑制细胞Ca²⁺内流有关。

关键词: 紫菀乙醇提取物; 豚鼠; 气管平滑肌; 张力变化

Abstract: The aim of the experiment was to study the effect of alcohol extract of Aster tataricus L.f.on the contraction of isolated guinea pig tracheal smooth muscle.The guinea pig tracheal smooth muscle was prepared by isolated tracheal thermostatic perfusion method.The effects of Aster tataricus L.f.on tracheal smooth muscle under the resting state and contraction stimulated by acetylcholine (Ach),histamine (His),CaCl₂,Ca²⁺ release and influx in cells without calcium were measured by BL-420F biological function experiment systems.The results showed that Aster tataricus L.f.could induce the contraction of isolated trachea at resting state at a low concentrations of 0.002 to 0.008 g/mL and exerted a relaxation on the isolated trachea when at the higher concentrations of 0.008 to 0.196 g/mL.All of the three concentrations of Aster tataricus L.f.could inhibit the tension stimulated by Ach,His and CaCl₂ which were antispasmodic agents,led to the non-parallelled rightward moving of cumulative concentration-response curve and the decline of maximal responses.The suppressive effect on His was the strongest,followed by Ach and CaCl₂,and the suppression could counteract the contraction induced by extracellular Ca²⁺influx significantly,and they were all expressed concentration-dependent manner.These results indicated the alcohol extract of Aster tataricus L.f.could relax tracheal smooth muscle by acting on M-receptor and histamine receptor and blocking Ca²⁺ passage,thus inhibiting extracellular Ca²⁺influx.

Key words: alcohol extract of Aster tataricus L.f.; guinea pig; tracheal smooth muscle; changes of tension

中图分类号:

S853.74

彭文静, 辛蕊华, 刘艳, 罗永江, 王贵波, 罗超应, 谢家声, 李锦宇, 郑继方. 紫菀乙醇提取物对豚鼠离体气管平滑肌收缩功能的影响[J]. , 2016, 43(6): 1572-1578.

PENG Wen-jing, XIN Rui-hua, LIU Yan, LUO Yong-jiang, WANG Gui-bo, LUO Chao-ying, XIE Jia-sheng, LI Jin-yu, ZHENG Ji-fang. Effects of Alcohol Extract of Aster tataricus L.f.on the Contraction of Guinea Pig Tracheal Smooth Muscle in vitro[J]. , 2016, 43(6): 1572-1578.

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紫菀乙醇提取物对豚鼠离体气管平滑肌收缩功能的影响

Effects of Alcohol Extract of Aster tataricus L.f.on the Contraction of Guinea Pig Tracheal Smooth Muscle in vitro

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