动物细胞流加培养工艺研究进展

doi:10.16431/j.cnki.1671-7236.2016.01.026

›› 2016, Vol. 43 ›› Issue (1): 176-181.doi: 10.16431/j.cnki.1671-7236.2016.01.026

动物细胞流加培养工艺研究进展

骆海燕¹, 窦冰然², 姜开维¹, 洪厚胜^1,3

1. 南京工业大学生物与制药工程学院, 南京 211816;
2. 南京工业大学化学与分子工程学院, 南京 210009;
3. 南京汇科生物工程设备有限公司, 南京 210009

收稿日期:2015-07-14 出版日期:2016-01-20 发布日期:2016-01-23
通讯作者: 洪厚胜 E-mail:hhs@njtech.edu.cn
作者简介:骆海燕(1991-),女,江苏盐城人,硕士,研究方向:动物细胞培养,E-mail:18260038159@163.com
基金资助:
国家高技术研究发展计划(863计划);生物过程关键技术及装备开发(2012AA021201)

Research Progress on Technology of Fed-batch in Animal Cell Culture

LUO Hai-yan¹, DOU Bing-ran², JIANG Kai-wei¹, HONG Hou-sheng^1,3

1. College of Life Science and Pharmaceutical Engineering, Nanjing Tech University, Nanjing 211816, China;
2. College of Chemistry and Molecular Engineering, Nanjing Tech University, Nanjing 210009, China;
3. State of Key Laboratory of Materials-oriented Chemical Engineering, Nanjing 210009, China

Received:2015-07-14 Online:2016-01-20 Published:2016-01-23

摘要/Abstract

摘要： 流加培养技术是动物细胞大规模生产中主流培养技术,被广泛应用于生物制品的生产,推动了生物技术产业的发展。流加培养过程中,需合理地向细胞持续提供所需营养物质,以满足其生长代谢和产物合成所需,控制代谢副产物的积累,缓解营养物耗竭和代谢副产物积累之间的矛盾。通过对流加培养的特性分析引出其优势,从培养过程中细胞代谢及基因调控、培养工艺的优化及流加过程的检测与控制等方面进行了阐述,最后提出了现如今流加培养技术存在的问题并进行了展望。

关键词: 动物细胞流加培养技术; 细胞代谢; 培养基; 控制策略

Abstract: Nowadays, fed-batch culture becomes the predominant mode of the large-scale animal cell culture process.It is widely used in the production of biological products, and promotes the development of the modem biological industry.In the process of culture, the cells should be provided with nutrient for its metabolism and products synthesis.The by-produces of the cells should also be controlled to relief the contradiction between nutrient consumption and by-produces accumulation.The advantage of fed-batch culture is drawn forth from analyzing the characteristics.In this paper, we reviewed the cell metabolism coupled with gene regulation, the optimization of culture process and control strategies linked with on-line monitor in the fed-batch culture process.On the basis of above-mentioned survey, the existent problems and prospects for animal cell cultivation were discussed.

Key words: fed-batch animal cell culture technology; cellular metabolism; medium; control strategy

中图分类号:

Q813.1⁺1

骆海燕, 窦冰然, 姜开维, 洪厚胜. 动物细胞流加培养工艺研究进展[J]. , 2016, 43(1): 176-181.

LUO Hai-yan, DOU Bing-ran, JIANG Kai-wei, HONG Hou-sheng. Research Progress on Technology of Fed-batch in Animal Cell Culture[J]. , 2016, 43(1): 176-181.

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动物细胞流加培养工艺研究进展

Research Progress on Technology of Fed-batch in Animal Cell Culture

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