冷刺激下AA肉鸡FBP1基因表达量的研究

doi:10.16431/j.cnki.1671-7236.2015.12.006

›› 2015, Vol. 42 ›› Issue (12): 3146-3152.doi: 10.16431/j.cnki.1671-7236.2015.12.006

冷刺激下AA肉鸡FBP1基因表达量的研究

刘火¹, 杜晓彤¹, 闫辰¹, 包军², 李剑虹¹

1. 东北农业大学生命科学学院, 哈尔滨 150030;
2. 东北农业大学动物科学技术学院, 哈尔滨 150030

收稿日期:2015-05-19 出版日期:2015-12-20 发布日期:2015-12-30
通讯作者: 李剑虹 E-mail:jhli@neau.edu.cn
作者简介:刘火(1991-),女,辽宁辽阳人,硕士,研究方向:动物行为与福利,E-mail:firefire76@163.com
基金资助:
黑龙江省自然基金项目"鸡慢性应激模型的建立及应激相关基因的筛选"(C200932)

Study on the Expression of FBP1 Gene in AA Broilers under Cold Stimulating Environment

LIU Huo¹, DU Xiao-tong¹, YAN Chen¹, BAO Jun², LI Jian-hong¹

1. College of Life Science and Technology, Northeast Agricultural University, Haerbin 150030, China;
2. College of Animal Science and Technology, Northeast Agricultural University, Haerbin 150030, China

Received:2015-05-19 Online:2015-12-20 Published:2015-12-30

摘要/Abstract

摘要： 为了解冷刺激条件下果糖-1,6-二磷酸酶1(FBP1)基因在肉鸡组织中的表达情况,试验选择75只AA肉鸡随机分成了对照组(正常饲养温度)和4个处理组(比正常饲养温度低3 ℃),从雏鸡8日龄开始每天分别进行1、3、5和24 h的冷刺激,在21日龄时结束冷刺激,并于22日龄屠宰测定FBP1基因在AA肉鸡各组织中的表达情况。结果表明,在冷刺激条件下,FBP1基因表达量存在组织特异性,在1 h处理组中FBP1基因在肝脏中的表达量显著高于其他各组织(P< 0.05),在3 h处理组中FBP1基因在肺脏中表达量显著高于除肝脏外的其他各组织(P< 0.05),在5 h处理组中FBP1基因在肝脏中的表达量显著高于其他各组织(P< 0.05),在24 h处理组中FBP1基因在胸腺中的表达量明显高于其他各组织(P< 0.05)。在相同组织不同处理组中,心脏FBP1基因的表达量除3 h组明显降低外,其余处理组随冷刺激时间延长呈下降趋势,肝脏中FBP1基因表达量在5 h组中显著高于其他各处理组(P< 0.05),脾脏中FBP1基因的表达量除在1 h组中明显降低外,其余各处理组随冷刺激时间延长呈下降趋势,肺脏中FBP1基因的表达量呈先上升后下降的趋势,肾脏中FBP1基因表达量除在24 h组中明显降低外,其余各组没有明显变化,胸腺中24 h组FBP1基因表达量显著高于其他处理组(P< 0.05),且其他处理组与对照组差异不显著(P >0.05)。

关键词: AA肉鸡; 冷刺激; FBP1基因; 表达

Abstract: In order to study of FBP1 gene expression under cold stimulating environment,75 AA broilers as research object were selected and randomly assigned to 5 groups,one of group was control group that raising in normal temperature,the remaining four groups were cold stimulation groups that raising temperature was 3 ℃ lower than the control group.The time of cold stimulation in four groups were 1,3,5 and 24 h,respectively.Cold stimulation started at 8 days of age,ended at 21 days of age.Broilers were slaughtered at 22 days of age and investigated FBP1 gene expression in each group.The results showed that the expression of FBP1 gene existed tissue specificity under cold stimulating environment.The relative expression level of FBP1 gene in liver was significantly higher than the other tissues in 1 h group (P< 0.05);The relative expression level of FBP1 gene in lung were significantly higher than the other tissues except of liver in 3 h group except for liver (P< 0.05);The relative expression level of FBP1 gene in liver were significantly higher than the other tissues in 5 h group (P< 0.05);The relative expression level of FBP1 gene in thymus were significantly higher than the other tissues in 24 h group (P< 0.05).FBP1 gene expression inside the heart was on the decline in the same organization different treatment groups as the rime of cold stimulating was increasing except for 3 h group.FBP1 gene expression of liver in 5 h group was significantly higher than other FBP1 gene expression in each treatment group.FBP1 gene expression of spleen was significant decreased in 1 h group,the other group extend a downward trend with the extension of cold stimulation time.FBP1 gene expression in lung was downward trend after rising first.FBP1 gene expression in kidney was significantly decreased in 24 h group,and the other groups had no obvious change.FBP1 gene expression of thymus in 24 h group was significantly higher than the other groups (P< 0.05),and there was no significant difference between control group and other four terament groups (P >0.05).

Key words: AA broiler; cold stimulation; FBP1 gene; expression

中图分类号:

Q786

刘火, 杜晓彤, 闫辰, 包军, 李剑虹. 冷刺激下AA肉鸡FBP1基因表达量的研究[J]. , 2015, 42(12): 3146-3152.

LIU Huo, DU Xiao-tong, YAN Chen, BAO Jun, LI Jian-hong. Study on the Expression of FBP1 Gene in AA Broilers under Cold Stimulating Environment[J]. , 2015, 42(12): 3146-3152.

参考文献

[1] Lin H,Decuypere E,Buyse J.Oxidative stress induced by corticosterone administration in broiler chickens (Gallus gallus domesticus):1.Chronic exposure[J].Comp Biochem Physiol B Biochem Mol Biol,2004,139(4):737-744.
[2] 屠云洁,耿照玉,陈国宏,等.动物冷应激反应中多种基因调控机制的研究[J].中国畜牧兽医,2010,37(1):84-87.
[3] 李丽娟,王安,芦燕.冷应激对肉仔鸡生长性能、免疫及抗氧化功能的影响[J].中国饲料,2009,17:42-43.
[4] Agrawal A,Jaggi A S,Singh N.Pharmacological investigations on adaptation in rats subjected to cold water immersion stress[J].Physiology & Behavior,2011,103(3-4):321-329.
[5] Nagamatsu S,Nakamichi Y,Yamamura C,et al.Decreased expression of t-SNARE,syntaxin 1,and SNAP-25 in pancreatic beta-cells is involved in impaired insulin secretion from diabetic GK rat islets:Restoration of decreased t-SNARE proteins improves impaired insulin secretion[J]. Diabetes,1999,48(12):2367-2373.
[6] Bell G I,Polonsky K S.Diabetes mellitus and genetically programmed defects in beta-cell function[J]. Nature,2002,414(6865):788-791.
[7] Matschinsky F M,Glaser B,Magnuson M A.Pancreatic beta-cell glucokinase:Closing the gap between theoretical concepts and experimental realities[J].Diabetes,1998,47(3):307-315.
[8] 李占梅,别建波,宋宏锐.果糖-1,6-二磷酸酶AMP变构抑制剂的研究进展[J].药学学报,2001,46(11):1291-1300.
[9] 马文涛,来启,林玉梅.应激对大鼠血清皮质醇及白细胞介素2、6、8水平的影响[J].中国心理卫生杂志,2002,16(1):14-15.
[10] 罗海吉,吉雁鸿,张云山,等.高温应激下补充L-精氨酸对小鼠免疫功能的影响[J].氨基酸和生物资源,2002,1:35-38.
[11] 张雷.运输应激和禁食应激对AA肉鸡组织结构的影响及相关基因表达的研究[D].哈尔滨:东北农业大学,2013.
[12] 杨莉,张莉,齐亚银,等.冷应激对湖羊血清因子及热休克蛋白70 mRNA表达的影响[J].中国畜牧兽医,2015,42(4):890-895.
[13] Hangalapura B N.Cold stress equally enhances in vivo pro-inflammatory cytokine gene expression in chicken lines divergently selected for antibody responses[J].Developmental & Comparative Immunology,2006,30(5):503-511.

[1]	韦明邦, 边巴琼达, 肖青青, 段梦琪, 强巴央宗, 商鹏. 藏猪CDKN1B基因克隆、生物信息学及表达分析[J]. 中国畜牧兽医, 2023, 50(6): 2196-2206.
[2]	肖鹏, 尚江华, 杨春艳, 李孟琪, 段安琴, 马小娅, 冯超, 黄晨茜, 张博, 周金陈, 韦科龙, 郑威, 郑海英. α-亚麻酸对水牛卵巢颗粒细胞体外培养的影响[J]. 中国畜牧兽医, 2023, 50(6): 2380-2387.
[3]	焦琳琳, 成玉婷, 吴庆国, 吴双, 吴植, 朱善元, 钱莺娟. 鸭坦布苏病毒Capsid蛋白原核表达及多克隆抗体制备[J]. 中国畜牧兽医, 2023, 50(6): 2395-2402.
[4]	李灿, 李楚楚, 曾维, 张宁, 纪春晓, 陈韬. 猪RegⅢγ蛋白的重组表达及功能研究[J]. 中国畜牧兽医, 2023, 50(6): 2414-2426.
[5]	宋越, 苏胜杰, 张帆, 白帆, 戴伶俐, 王娜, 王大伟, 杨茜雯, 赵世华, 张月梅. 溶血性曼氏杆菌截短型白细胞毒素的原核表达及多克隆抗体制备[J]. 中国畜牧兽医, 2023, 50(6): 2479-2486.
[6]	欧云文, 潘琴, 汪洋, 代军飞, 任绍科, 张洋, 张杰. 猪细小病毒6型ORF2基因的截短表达及多克隆抗体制备[J]. 中国畜牧兽医, 2023, 50(6): 2487-2495.
[7]	王浩睿, 禹保军, 杨嘉翊, 魏榕, 付希, 王川川, 张迪, 李德生, 蔡正云, 顾亚玲, 张娟. 静原鸡DERA基因生物信息学分析及组织表达研究[J]. 中国畜牧兽医, 2023, 50(5): 1764-1773.
[8]	潘鹏丞, 雷宗全, 李显, 胡湘云, 秦谦涛, 覃兆鲜, 关志惠, 陈宝剑, 谢炳坤. 陆川猪MYL2基因生物信息学分析、真核表达载体构建及组织表达研究[J]. 中国畜牧兽医, 2023, 50(5): 1796-1806.
[9]	卓娜, 朱忠武, 李静, 胡世享, 王建昌, 林华, 陈朝林, 韩佃刚, 艾军, 陈培富. 猪源戊型肝炎病毒ORF2蛋白生物信息学分析及真核表达研究[J]. 中国畜牧兽医, 2023, 50(5): 1959-1970.
[10]	张芳源, 蔺雅婷, 杨大为, 陈虎, 李桂梅, 单虎. 非洲猪瘟病毒P30蛋白原核表达及间接ELISA检测方法建立[J]. 中国畜牧兽医, 2023, 50(5): 2012-2022.
[11]	王雪平, 张孝俊, 李晓月, 王国栋, 张福良, 宋玉伟, 张明亮, 马磊. 高致病性禽腺病毒血清4型Hexon蛋白的原核表达及多克隆抗体的制备[J]. 中国畜牧兽医, 2023, 50(5): 2053-2060.
[12]	陶庆华, 黄彩云, 陈超, 刘威, 孙宁宁, 李昂. 白羽番鸭LIF基因克隆及其组织表达差异分析[J]. 中国畜牧兽医, 2023, 50(4): 1296-1306.
[13]	刘红润, 朱思燃, 奉玲丽, 张坤, 颜港, 王钰滨, 张帅, 江山, 许迪, 兰干球, 梁晶. 大白猪CDO1基因克隆、生物信息学分析及组织表达定位[J]. 中国畜牧兽医, 2023, 50(4): 1352-1363.
[14]	张哲玮, 代小童, 韦祖丹, 陈云鹏, 甘晶, 贝为成. 猪多杀性巴氏杆菌OmpW、TbpA蛋白的原核表达及免疫原性研究[J]. 中国畜牧兽医, 2023, 50(4): 1489-1498.
[15]	胡乐玉, 胡欣瑶, 李颖, 赵盛淼, 沈凤臻, 王长法, 李亮亮, 王彤彤, 任慧英. 德州驴HO-1基因生物信息学分析及多克隆抗体制备[J]. 中国畜牧兽医, 2023, 50(4): 1499-1510.

冷刺激下AA肉鸡FBP1基因表达量的研究

Study on the Expression of FBP1 Gene in AA Broilers under Cold Stimulating Environment

可视化

摘要/Abstract

引用本文

使用本文

参考文献

相关文章 15

编辑推荐

Metrics

本文评价