3种中草药对断奶仔猪小肠形态学影响的比较研究

doi:10.16431/j.cnki.1671-7236.2015.09.036

《中国畜牧兽医》---唯一指定的官方网站 ›› 2015, Vol. 42 ›› Issue (9): 2459-2466.doi: 10.16431/j.cnki.1671-7236.2015.09.036

3种中草药对断奶仔猪小肠形态学影响的比较研究

李慧峰¹, 李清宏¹, 李宏全¹, 郝瑞荣¹, 陈建辉¹, 谷建文²

1. 山西农业大学动物科技学院, 太谷 030801;
2. 山东省利津县动物疫病防治监控中心, 利津 257400

收稿日期:2015-03-09 出版日期:2015-09-20 发布日期:2015-09-25
通讯作者: 李清宏 E-mail:sxaulqh88@126.com
作者简介:李慧峰(1989-),女,山西长治人,硕士生,研究方向:农业推广,E-mail:cdwslhfd@163.com
基金资助:
山西省科技攻关项目(20110311033);山西农业大学人才引进项目(2013YJ16)

Comparison Study on Effects of Three Chinese Herbal Medicine on Intestinal Morphology in Weaning Piglets

LI Hui-feng¹, LI Qing-hong¹, LI Hong-quan¹, HAO Rui-rong¹, CHEN Jian-hui¹, GU Jian-wen²

1. College of Animal Science and Veterinary Medicine, Shanxi Agricultural University, Taigu 030801, China;
2. Lijin County Animal Disease Prevention and Control Monitoring Center, Lijin 257400, China

Received:2015-03-09 Online:2015-09-20 Published:2015-09-25

摘要/Abstract

摘要： 本研究旨在比较神曲、麦芽、山楂3种助消化中草药对断奶仔猪小肠不同部位组织形态学的影响。本试验以杜长大三元猪为研究对象,选取40头28日龄、体重为(8.13±1.32)kg断奶仔猪,添加1%的中草药饲喂,分空白组、神曲组、麦芽组、山楂组,每组两个重复,每重复5头,分别在试验第7和14天早晨空腹屠宰取样,经HE染色,200×显微镜下照相,用Image-Pro Plus软件测定小肠各段绒毛高度(VH)、隐窝深度(CD)、肠壁厚度(IWT)、绒毛高度与隐窝深度的比值(V/C)。结果显示,神曲组在回肠段的IWT显著或极显著高于空白组(P<0.05;P<0.01);第7天时山楂组的十二指肠和空肠的VH和IWT均极显著低于空白组(P<0.01),回肠段的IWT和第14天回肠段VH均极显著高于空白组(P<0.01);第7天的麦芽组十二指肠和空肠VH及空肠IWT均极显著低于空白组(P<0.01),第7天的回肠段CD及十二指肠和回肠段IWT均极显著高于空白组(P<0.01)。不同单味中草药对仔猪小肠不同部位组织形态学的影响不同,源于肠道消化物质的功能不同,对十二指肠、空肠、回肠影响最大的分别是麦芽、山楂、神曲。

关键词: 神曲; 山楂; 麦芽; 小肠形态

Abstract: The objective of this study was to compare the effect of three Chinese herbal medicine (medicated leaven, hawthorn, fructus hordei germinates) on histomorphology of different parts of small intestine in weaning piglets.40 28-day old ternary pigs (Duroc×Landrace×Yorkshire) with the body weight (8.13±1.32)kg were divided into four groups, control group, medicated leaven group, hawthorn group and fructus hordei germinates group, and feeding with 1% Chinese herbal medicine, there were two repeats in each group and each repeat had 5 piglets, slaughtering and sampling at 7th and 14th day morning, respectively, sections were used for HE staining.The staining tissues were taken pictures under a microscope camera (200×) and measured VH, CD, IWT and V/C in different parts of small intestine with Image-Pro Plus.The result showed that the IWH of ileum in medicated leaven group was significantly or extremely significantly higher than control group (P<0.05;P<0.01).The VH and IWT of duodenum and jejunum at 7th day in hawthorn group were extremely significantly lower than control group (P<0.01);The IWT of ileum and the VH of ileum at 14th day were extremely significantly higher than control group (P<0.01).In fructus hordei germinates group, the VH of duodenum and jejunum at 7th day and IWT of jejunum were extremely significantly lower than control group (P<0.01);The CD of ileum at 7th day, the IWT of duodenum and ileum at 7th day were extremely significantly higher than control group (P<0.01).The effects of different simple Chinese herbal medicine on histomorphology of different parts of small intestine in piglets were different, because of the different digestive functions of small intestine, the most effects of Chinese herbal medicine an duodenum, jejunum and ileum were fructus hordei germinates, medicated leaven and hawthorn, respectively.

Key words: medicated leaven; hawthorn; fructus hordei germinates; small intestinal morphology

中图分类号:

S859.7

李慧峰, 李清宏, 李宏全, 郝瑞荣, 陈建辉, 谷建文. 3种中草药对断奶仔猪小肠形态学影响的比较研究[J]. 《中国畜牧兽医》---唯一指定的官方网站, 2015, 42(9): 2459-2466.

LI Hui-feng, LI Qing-hong, LI Hong-quan, HAO Rui-rong, CHEN Jian-hui, GU Jian-wen. Comparison Study on Effects of Three Chinese Herbal Medicine on Intestinal Morphology in Weaning Piglets[J]. , 2015, 42(9): 2459-2466.

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3种中草药对断奶仔猪小肠形态学影响的比较研究

Comparison Study on Effects of Three Chinese Herbal Medicine on Intestinal Morphology in Weaning Piglets

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