关键词: Cchl1a3; 基因敲入; 电解质; 可行性

Abstract: In this experiment,dry chemical method and wet chemical method were used to detect electrolyte levels of Cchl1a3-R528H gene knock-in mice,we compared the two different detection methods and analyzed their correlation.Firstly,we tested the precision of the electrolyte analyzer,and then selected the same mouse,collected the whole blood from tail with dry chemical method to detect electrolyte levels and collected the whole blood from the retro-orbital venous plexus with wet chemical method to detect electrolyte levels,and compared the two different detection methods.The results showed that the CV of Na⁺,Cl^- and K⁺ detected by dry electrolyte analyzer were(2.06±0.18)%,(3.61±0.22)% and (4.40±0.19)%,respectively,all < 4.5%.Compared with the results detected by two different detection methods above-mentioned,the difference was not statistically significant (P> 0.05),and there were linear positive correlations between the two detection methods in Na⁺,Cl^- and K⁺(r of Na⁺ and Cl^- were both > 0.975).The result indicated that the dry electrolyte analyzer had high precision and good stability.The detected electrolytes results by two detection methods were with high correlation and good comparability,subsequent experiments could use dry chemical method to replace wet chemical method to detect electrolytes.

Key words: Cchl1a3; gene knock-in; electrolyte; feasibility