波尔山羊线粒体融合蛋白2基因的克隆及序列分析

doi:10.16431/j.cnki.1671-7236.2015.05.006

›› 2015, Vol. 42 ›› Issue (5): 1076-1081.doi: 10.16431/j.cnki.1671-7236.2015.05.006

波尔山羊线粒体融合蛋白2基因的克隆及序列分析

叶锦辉, 张凯照, 于梦, 王晴楠, 刘健新, 陶攀, 宁章勇

华南农业大学兽医学院, 广州 510642

收稿日期:2014-10-29 出版日期:2015-05-20 发布日期:2015-06-02
通讯作者: 宁章勇 E-mail:ningzhyong@126.com
作者简介:叶锦辉(1988-),男,广东肇庆人,硕士,研究方向:兽医病理学,E-mail:yejinhuiorfei@163.com
基金资助:
广东省自然科学基金(s2013010013041)

Cloning and Sequence Analysis of MFN2 Gene from Boer Goat

YE Jin-hui, ZHANG Kai-zhao, YU Meng, WANG Qing-nan, LIU Jian-xin, TAO Pan, NING Zhang-yong

College of Veterinary Medicine, South China Agricultural University, Guangzhou 510642, China

Received:2014-10-29 Online:2015-05-20 Published:2015-06-02

摘要/Abstract

摘要： 线粒体融合蛋白2(mitofusin-2,MFN2)是位于线粒体外膜上的跨膜蛋白,也是外膜的信号分子和药物作用靶蛋白,它在细胞增殖、分化、凋亡和多种疾病中起着重要作用。为深入研究山羊MFN2基因在炎症疾病过程中的分子作用机制,本试验采用RT-PCR的方法克隆了波尔山羊MFN2基因,并对其进行了序列测定及其编码蛋白的结构分析。结果显示,克隆得到的波尔山羊MFN2基因大小为2 441 bp,其编码的蛋白由705个氨基酸组成。波尔山羊MFN2基因种间同源性低,存在Fzo-mitofusin结构域、DLP-2结构域,是结构保守蛋白;存在1个潜在跨膜区,位于576—595位氨基酸(loop1);不存在信号肽。试验首次克隆了波尔山羊MFN2基因,为探索山羊MFN2基因与炎症发生之间关系的分子机制提供了基础数据。

关键词: 波尔山羊; 线粒体融合蛋白2(mitofusin-2,MFN2); 克隆; 序列分析

Abstract: Mitofusin-2 (MFN2), an outer transmembrane protein on the mitochondrion, which was a signaling and therapeutic target molecule, played a critical role in cell proliferation, differentiation, apoptosis and many diseases.For further researching the molecular mechanism of MFN2 gene in inflammatory disease, the MFN2 gene from Boer goat was cloned by RT-PCR, and its sequence and coding protein structure were analyzed.The results showed that the full length of MFN2 gene was 2 441 bp and its coding protein was composed of 705 amino acids.It was low homology between species with conservative protein structure, Fzo-mitofusin structural domain and DLP-2 structural domain, respectively.The MFN2 had a potential transmembrane domain, and was consist of 576 to 595 amino acid without signal peptide.It was the first time to clone MFN2 gene from Boer goat, which provided basic data for exploring the molecular mechanism of the relationship between MFN2 gene from Boer goat and inflammation.

Key words: Boer goat; mitofusin-2 (MFN2); clone; sequence analysis

中图分类号:

叶锦辉, 张凯照, 于梦, 王晴楠, 刘健新, 陶攀, 宁章勇. 波尔山羊线粒体融合蛋白2基因的克隆及序列分析[J]. , 2015, 42(5): 1076-1081.

YE Jin-hui, ZHANG Kai-zhao, YU Meng, WANG Qing-nan, LIU Jian-xin, TAO Pan, NING Zhang-yong. Cloning and Sequence Analysis of MFN2 Gene from Boer Goat[J]. , 2015, 42(5): 1076-1081.

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波尔山羊线粒体融合蛋白2基因的克隆及序列分析

Cloning and Sequence Analysis of MFN2 Gene from Boer Goat

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