microRNA let-7调控动物个体发育的研究进展

doi:10.16431/j.cnki.1671-7236.2015.02.016

摘要/Abstract

摘要： microRNA(miRNA)是一类广泛存在于多细胞动物中的进化保守的大小为18~25 nt的非编码小分子RNA,可以通过与靶基因mRNA的非编码区(3'UTR)结合导致mRNA降解,或阻断mRNA翻译而调节基因表达。let-7是在线虫中发现的具有转录后调节功能的小分子RNA,具有高度的保守性,研究发现,let-7参与动物个体多个器官组织的发育过程。作者综述了近年来let-7参与调控脑、神经系统、心肺系统和肌肉发育等组织器官的研究成果,初步阐述了let-7调控组织器官发育的作用机制,以期为进一步探索let-7在动物体内的功能奠定基础。

关键词: microRNA; let-7; 个体发育; 组织器官

Abstract: microRNAs (miRNAs) are evolutionary conserved small noncoding RNAs of 18 to 25 nt that were found in all metazoans,and it regulates gene expression by base pairing with their target mRNA,leading to mRNA cleavage or translational repression.let-7 is a small RNA molecule originally found in worms,and it is highly conserved and plays a vital role in posttranscriptional regulation.The study found that let-7 takes part in the development of organs and tissues.This review summarized the recent progresses about the function of let-7 miRNA in the development of brain,nervous system,lung and cardiovascular system,and muscle development,and elucidated the possible mechanism of regulating the developmental processes of animal tissues and organs.It will provide the basis for further exploring the function of let-7 miRNA in animals.

Key words: microRNA; let-7; individual development; tissue and organ

中图分类号:

Q522

赵拴平, 贾玉堂, 徐磊, 阮永明. microRNA let-7调控动物个体发育的研究进展[J]. , 2015, 42(2): 342-346.

ZHAO Shuan-ping, JIA Yu-tang, XU Lei, RUAN Yong-ming. Progress of Animal Individual Development Regulated by microRNA Let-7[J]. , 2015, 42(2): 342-346.

参考文献

[1] Reinhart B J,Slack F J,Basson M,et al.The 21-nucleotide let-7 RNA regulates developmental timing in Caenorhabditis elegans[J].Nature,2000,403(6772):901-906.
[2] Ruby J G,Jan C,Player C,et al.Large-scale sequencing reveals 21U-RNAs and additional microRNAs and endogenous siRNAs in C.elegans[J]. Cell,2006,127(6):1193-1207.
[3] Pasquinelli A E,Reinhart B J,Slack F,et al.Conservation of the sequence and temporal expression of let-7 heterochronic regulatory RNA[J]. Nature,2000,408(6808):86-89.
[4] 王虹,滕鸿琦,施珏平,等.let-7 microRNA调控动物器官发育的研究进展[J].生命科学,2011,23(4):364-368.
[5] Caygill E E,Johnston L A.Temporal regulation of metamorphic processes in drosophila by the let-7 and miR-125 heterochronic microRNAs[J].Curr Biol,2008,18(13):943-950.
[6] Sokol N S,Xu P,Jan Y N,et al.Drosophila let-7 microRNA is required for remodeling of the neuromusculature during metamorphosis[J].Genes Dev,2008,22(12):1591-1596.
[7] Huang J C,Babak T,Corson T W,et al.Using expression profiling data to identify human microRNA targets[J].Nat Methods,2007,4(12):1045-1049.
[8] Johnson C D,Esquela-Kerscher A,Stefani G,et al.The let-7 microRNA represses cell proliferation pathways in human cells[J].Cancer Res,2007,67(16):7713-7722.
[9] Legesse-Miller A,Elemento O,Pfau S J,et al.let-7 overexpression leads to an increased fraction of cells in G2/M,direct down-regulation of Cdc34,and stabilization of Wee1 kinase in primary fibroblasts[J]. J Biol Chem,2009,284(11):6605-6609.
[10] Cocklin R,Heyen J,Larry T,et al.New insight into the role of the Cdc34 ubiquitin-conjugating enzyme in cell cycle regulation via Ace2 and Sic1[J]. Genetics,2011,187(3):701-715.
[11] Takamizawa J,Konishi H,Yanagisawa K,et al.Reduced expression of the let-7 microRNAs in human lung cancers in association with shortened postoperative survival[J].Cancer Research,2004,64(11):3753-3756.
[12] Dahiya N,Sherman-Baust C A,Wang T L,et al.microRNA expression and identification of putative miRNA targets in ovarian cancer[J].PLoS One,2008,3(6):e2436.
[13] O'Hara A J,Wang L,Dezube B J,et al.Tumor suppressor microRNAs are underrepresented in primary effusion lymphoma and Kaposi sarcoma[J].Blood,2009,113(23):5938-5941.
[14] Johnson S M,Grosshans H,Shingara J,et al.RAS is regulated by the let-7 microRNA family[J]. Cell,2005,120(5):635-647.
[15] Kumar M S,Erkeland S J,Pester R E,et al.Suppression of non-small cell lung tumor development by the let-7 microRNA family[J].PNAS,2008,105(10):3903-3908.
[16] Lee Y S,Dutta A.The tumor suppressor microRNA let-7 represses the HMGA2 oncogene[J]. Genes Dev,2007,1(9):1025-1030.
[17] Sampson V B,Rong N H,Han J,et al.microRNA let-7a down-regulates MYC and reverts MYC-induced growth in Burkitt lymphoma cells[J].Cancer Res,2007,67(20):9762-9770.
[18] 王丽,王媛媛,刘海莉,等.microRNA与神经系统发育的研究进展[J].中南大学学报(医学版),2013,38(3):323-328.
[19] Kucherenko M M,Barth J,Fiala A,et al.Steroid-induced microRNA let-7 acts as a spatio-temporal code for neuronal cell fate in the developing drosophila brain[J]. EMBO J,2012,31(24):4511-4523.
[20] Le M T,Xie H,Zhou B,et al.microRNA-125b promotes neuronal differentiation in human cells by repressing multiple targets[J].Mol Cell Biol,2009,29(19):5290-5305.
[21] Wulczy F G,Smirnova L,Rybak A,et al.Post-transcriptional regulation of the let-7 microRNA during neural cell specification[J].FASEB J,2007,21(2):415-426.
[22] Zhao C,Sun G,Li S,et al.microRNA let-7b regulates neural stem cell proliferation and differentiation by targeting nuclear receptor TLX signaling[J].Proc Natl Acad Sci USA,2010,107(5):1876-1881.
[23] Cao L,Kong L P,Yu Z B,et al.microRNA expression profiling of the developing mouse heart[J].Int J Mol Med,2012,30(5):1095-1104.
[24] Bao M H,Feng X,Zhang Y W,et al.Let-7 in cardiovascular diseases,heart development and cardiovascular differentiation from stem cells[J].Int J Mol Sci,2013,14(11):23086-23102.
[25] Luo X,Zhang H,Xiao J,et al.Regulation of human cardiac ion channel genes by microRNAs:Theoretical perspective and pathophysiological implications[J].Cell Physiol Biochem,2010,25(6):571-586.
[26] Ikeda S,Kong S W,Lu J,et al.Altered microRNA expression in human heart disease[J].Physiol Genomics,2007,31(3):367-373.
[27] Shi G,Cui Q,Zhang Y.microRNA set:A novel way to uncover the potential black box of chronic heart failure in microRNA microarray analysis[J].J Comput Sci Syst Biol,2009,2:240-246.
[28] Harris K S,Zhang Z,McManus M T,et al.Dicer function is essential for lung epithelium morphogenesis[J].Proc Natl Acad Sci USA,2006,103(7):2208-2213.
[29] Harfe B D,McManus M T,Mansfield J H,et al.The RNase Ⅲ enzyme Dicer is required for morphogenesis but not patterning of the vertebrate limb[J].Proc Natl Acad Sci USA,2005,102(31):10898-10903.
[30] Papaioannou G,Inloes J B,Nakamura Y,et al.let-7 and miR-140 microRNAs coordinately regulate skeletal development[J].Proc Natl Acad Sci USA,2013,110(35):E3291-E3300.
[31] Jiang L Q,Franck N,Egan B,et al.Autocrine role of interleukin-13 on skeletal muscle glucose metabolism in type 2 diabetic patients involves microRNA let-7[J].Am J Physiol Endocrinol Metab,2013,305(11):E1359-E1366.
[32] 江林.重症肌无力中差异miRNA的筛选和let-7c分子作用机制研究[D].上海:华东理工大学,2013.
[33] Newman M A,Hammond S M.Lin-28:An early embryonic sentinel that blocks let-7 biogenesis[J].Int J Biochem Cell Biol,2010,42(8):1330-1333.
[34] Lehrbach N J,Armisen J,Lightfoot H L,et al.LIN-28 and the poly (U) polymerase PUP-2 regulate let-7 microRNA processing in Caenorhabditis elegans[J].Nat Struct Mol Biol,2009,16(10):1016-1020.
[35] Faehnle C R,Walleshauser J,Joshua-Tor L.Mechanism of Dis3l2 substrate recognition in the lin28-let-7 pathway[J].Nature,2014,514(7521):252-256.
[36] Sempere L F,Dubrovsky E B,Dubrovskaya V A,et al.The expression of the let-7 small regulatory RNA is controlled by ecdysone during metamorphosis in drosophila melanogaster[J].Dev Biol,2002,244(1):170-179.
[37] Mitra D,Das P M,Huynh F C,et al.Jumonji/ARID1 B (JARID1B) protein promotes breast tumor cell cycle progression through epigenetic repression of microRNA let-7e[J].J Biol Chem,2011,286(47):40531-40535.
[38] Sakamoto S,Aoki K,Higuchi T,et al.The NF90-NF45 complex functions as a negative regulator in the microRNA processing pathway[J].Mol Cell Biol,2009,29(13):3754-3769.