关键词: 猪博卡病毒; 非结构蛋白NP1; 克隆; 序列分析; 基因分型

Abstract: In order to demonstrate the nonstructural proteins 1 (NP1) molecular characterization,the target fragments was obtained from post-weaning multisystemic wasting syndrome piglets using PCR method with specific primers,targeting to the NP1 fragments.The results showed that the porcine bocavirus NP1 gene was 675 bp in length,coding an open reading frame (ORF) with 224 amino acids.The nucleotide sequence were compared with the porcine bocavirus NP1 downloaded from GenBank using MegAlign.The sequenced gene shared the highest homologies of nucleotides with the USA porcine bocavirus OH110 strain at 97.3%,and only shared 81.8% identity to IN109-1 strain; which shared the homogeneity with the United Kingdom porcine bocavirus F41 strain and 64-1 strain at 82.5% and 80.9%,respectively.However,the cloned sequence only shared the homologies of nucleotides with porcine bocavirus H18 strain at 43.8%.According to the phylogenetic tree,the porcine bocavirus could be divided into three genotypes,all of which the homologies of nucleotides were lower than 50.0%.The results suggested that the porcine bocavirus could be divided into three different genus in genus bocavirus.

Key words: porcine bocavirus; nonstructural proteins 1 (NP1); clone; sequence analysis; genotyping classification