应用PCR-DGGE分析鸭场土壤细菌群落结构

doi:10.16431/j.cnki.1671-7236.2015.01.010

›› 2015, Vol. 42 ›› Issue (1): 67-73.doi: 10.16431/j.cnki.1671-7236.2015.01.010

应用PCR-DGGE分析鸭场土壤细菌群落结构

李世静¹, 嵇辛勤^1,2, 文明^1,2, 陈佳琪¹, 刘廷江¹

1. 贵州大学动物科学学院, 贵阳 550025;
2. 贵州省动物疫病研究室, 贵阳 550025

收稿日期:2014-08-26 出版日期:2015-01-20 发布日期:2015-02-06
通讯作者: 嵇辛勤 E-mail:lishijing9289@163.com
作者简介:李世静(1990-), 女, 贵州遵义人, 硕士生, 研究方向:兽医公共卫生学, E-mail:lishijing9289@163.com
基金资助:
贵州省科技厅重大专项子课题(黔科合重大专项字[2012]6004);贵州省科技计划(黔科合NY[2013]3074号)

Analysis of Bacterial Community Structure in Soil Sample of Duck Farm by PCR-DGGE

LI Shi-jing¹, JI Xin-qin^1,2, WEN Ming^1,2, CHEN Jia-qi¹, LIU Ting-jiang¹

1. College of Animal Science, Guizhou University, Guiyang 550025, China;
2. Laboratory for Animal Disease of Guizhou, Guiyang 550025, China

Received:2014-08-26 Online:2015-01-20 Published:2015-02-06

摘要/Abstract

摘要： 为了解贵州省三穗县8个养鸭场环境土壤中细菌群落结构多样性概况,本试验采用试剂盒提取土壤样品细菌总DNA,PCR扩增细菌16S rDNA V3可变区,并通过变性梯度凝胶电泳(DGGE)技术对扩增产物进行凝胶分离,回收测序共得到43条特异性条带,其中7、8号养鸭场条带数相对较少.DGGE图谱经Quantity One软件分析,结果显示,同一鸭场样品相似性存在差异,绝大部分鸭场样品间相似性均高于50%,所测序列与GenBank中相应原核生物16S rDNA序列经同源性比对可见相似性在90%~100%之间,共包括5个大纲的细菌种类:变形菌门(Proteobacteria)的α,β类群、拟杆菌门(Bacteroidetes)、放线菌门(Actinobacteria)、酸杆菌门(Acidobacteria)和厚壁菌门(Firmicutes).

关键词: 变性梯度凝胶电泳; 鸭场; 土壤; 细菌群落结构

Abstract: To investigate the diversity of bacterial community structure in soil sample of 8 duck farms in Sansui Guizhou province, total DNA of the samples were extracted with kit, the bacterial 16S rDNA V3 variable sequence was amplified by PCR, the amplified sequence were separated by denaturing gradient gel electrophoresis (DGGE) technique, 43 bands were showed, number 7, 8 were relatively small.The DGGE fingerprint was analyzed by software Quantity One, and showed that the same duck farm similarities existed difference, while the similarity were higher than 50% of the majority.Results showed that the similarities between the sequences and sequences in GenBank were 90% to 100%, including α-Proteobacteria, β-Proteobacteria, Bacteroidetes, Actinobacteria, Acidobacteria and Firmicutes.

Key words: denaturing gradient gel electrophoresis; duck farm; soil; bacterial community structure

中图分类号:

S154.3

李世静, 嵇辛勤, 文明, 陈佳琪, 刘廷江. 应用PCR-DGGE分析鸭场土壤细菌群落结构[J]. , 2015, 42(1): 67-73.

LI Shi-jing, JI Xin-qin, WEN Ming, CHEN Jia-qi, LIU Ting-jiang. Analysis of Bacterial Community Structure in Soil Sample of Duck Farm by PCR-DGGE[J]. , 2015, 42(1): 67-73.

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应用PCR-DGGE分析鸭场土壤细菌群落结构

Analysis of Bacterial Community Structure in Soil Sample of Duck Farm by PCR-DGGE

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