脂多糖对猪繁殖与呼吸综合征病毒诱导的抗病毒免疫反应的影响

中国畜牧兽医

脂多糖对猪繁殖与呼吸综合征病毒诱导的抗病毒免疫反应的影响

王冬梅，冯亚琼，崔春晓，李娜娜，杜东颖，秦运杰，付朋飞，夏平安

河南农业大学牧医工程学院，河南郑州 450002

收稿日期:2014-04-08 出版日期:2014-08-20 发布日期:2014-08-22
通讯作者: 夏平安（1964—），男，湖北人，博士，教授。 E-mail: xpa88@163.com
作者简介:王冬梅（1987—），女，河南人，硕士生，研究方向：动物分子病毒学及分子免疫学。
基金资助:
国家自然科学基金（31172346）。

Effects of Lipopolysaccharide on Antiviral Immune Response Induced by Porcine Reproductive and Respiratory Syndrome Virus

WANG Dong-mei, FENG Ya-qiong, CUI Chun-xiao, LI Na-na, DU Dong-ying, QIN Yun-jie, FU Peng-fei, XIA Ping-an

College of Animal Husbandry and Veterinary, Henan Agricultural University, Zhengzhou 450002, China

Received:2014-04-08 Online:2014-08-20 Published:2014-08-22

摘要/Abstract

摘要： 为研究脂多糖对猪繁殖与呼吸综合征病毒(porcine reproductive and respiratory syndrome virus, PRRSV)诱导的抗病毒免疫反应的影响,本研究采用100 TCID₅₀的PRRSV感染猪肺巨噬细胞（porcine alveolar macrophage，PAM），感染12 h后用100 ng/mL脂多糖(lipopolysaccharide，LPS)处理感染细胞，分别培养12、24、36、48 h后收集细胞及上清，同时设PRRSV组、LPS组和PAM细胞组，用河南农业大学兽医微生物研究室已建立的Real-time qPCR方法对LPS+PRRSV组和PRRSV组中PRRSV复制水平及各组PAM细胞中IFN-α、TNF-α mRNA转录水平变化进行定量分析。结果显示，LPS+PRRSV组与PRRSV组相比，PRRSV拷贝数12～48 h均较低，IFN-α mRNA转录水平显著升高（P＜0.05），TNF-α mRNA转录水平升高，在48 h时转录水平降低。而与LPS组相比，LPS+PRRSV组IFN-α mRNA转录水平在12 h时升高，24、36、48 h均降低。结果表明，PRRSV感染PAM细胞经LPS刺激后，IFN-α、TNF-α mRNA转录水平显著升高（P＜0.05），从而抑制了PRRSV在PAM细胞内的复制。

关键词: 猪繁殖与呼吸综合征病毒; 猪肺泡巨噬细胞; IFN-α TNF-α 脂多糖

Abstract: To study the effects of lipopolyscaccharide on antiviral immune response induced by porcine reproductive and respiratory syndrome virus (PRRSV), porcine alveolar macrophage (PAM) cell was prepared and cultured with PRRSV solution 100 TCID₅₀. After inoculated 12 h, cells were treated with 100 ng/mL LPS. Then collected cells and supernatants after treated 12, 24, 36 and 48 h, respectively. The level of the virus, IFN-α and TNF-α mRNA in the PAM cells were quantitated by Real-time qPCR including lipopolysaccharide (LPS) stimulation of PRRSV infected PAM cells group, PRRSV infection control group, LPS stimulated cells control group and cell control group. The results showed that compared with unstimulated with LPS, the replication of PRRSV in PAM cells infected with PRRSV stimulated with LPS group was less than that of PRRSV infection control group from 12 to 48 h. The transcription level of IFN-α mRNA improved significantly （P＜0.05） and the transcription level of TNF-α mRNA improved and reduced at 48 h. Compared with LPS stimulation control group, LPS stimulation of PRRSV infected PAM cells group, IFN-α mRNA transcription level improved at 12 h and reduced at 24, 36 and 48 h. So PRRSV infected PAM cells stimulated with LPS, the transcription of IFN-α, TNF-α mRNA was significantly improved （P＜0.05）, thereby inhibiting the replication of PRRSV in PAM cells.

Key words: porcine reproductive and respiratory syndrome virus; porcine alveolar macrophage; IFN-α; TNF-α; lipopolysaccharide

王冬梅，冯亚琼，崔春晓，李娜娜，杜东颖，秦运杰，付朋飞，夏平安. 脂多糖对猪繁殖与呼吸综合征病毒诱导的抗病毒免疫反应的影响[J]. 中国畜牧兽医.

WANG Dong-mei, FENG Ya-qiong, CUI Chun-xiao, LI Na-na, DU Dong-ying, QIN Yun-jie, FU Peng-fei, XIA Ping-an. Effects of Lipopolysaccharide on Antiviral Immune Response Induced by Porcine Reproductive and Respiratory Syndrome Virus[J]. .

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