氟喹诺酮类药物胶体金试纸的研制

中国畜牧兽医

氟喹诺酮类药物胶体金试纸的研制

冯婷婷^1,2, 刘一兵^1,2, 侯惠仁^1,2, 贾娟娟^1,2, 李振华^1,2, 韩世泉²

1. 原子高科股份有限公司,北京 102413;
2. 中国原子能科学研究院同位素研究所,北京 102413

收稿日期:2012-02-01 出版日期:2012-09-20 发布日期:2012-09-18
通讯作者: 韩世泉(1960－),男,天津人,研究员,主要从事免疫分析技术研究。E-mail:han53@163.com;Tel:010-69358165 E-mail:han53@163.com
作者简介:冯婷婷(1981－),女,北京人,助理研究员,主要从事免疫分析技术研究。

Development of an Immunochromatographic Strip Test for Fluoroquinolones

FENG Ting-ting^1,2, LIU Yi-bing^1,2, HOU Hui-ren^1,2, JIA Juan-juan^1,2, LI Zhen-hua^1,2, HAN Shi-quan²

1. Beijing Atom HighTech Co., Ltd., Beijing 102413, China;
2. Department of Isotope,China Institute of Atomic Energy, Beijing 102413, China

Received:2012-02-01 Online:2012-09-20 Published:2012-09-18

摘要/Abstract

摘要： 用针对抗氟喹诺酮类(fluoroquinolones,FQs)药物的广谱性单克隆抗体建立了可以同时检测11种FQs药物的胶体金免疫层析方法。该试纸条用20 nm的胶体金标记单抗,将诺氟沙星－卵清蛋白喷涂在检测线上,羊抗小鼠二抗喷涂在质控线上。该胶体金试纸对猪肉和虾中环丙沙星、恩诺沙星和氧氟沙星的检测限都是30 ng/g,对诺氟沙星、培氟沙星、依诺沙星、麻保沙星、洛美沙星、达氟沙星、沙拉沙星和二氟沙星8种药物在以上两种样品中添加浓度为100 ng/g时都可被检出,整个检测过程包括样品前处理的时间可在20 min内完成。试验结果表明符合对这11种FQs药物在鸡肉和虾中残留现场大量筛查的要求。

关键词: 氟喹诺酮; 胶体金; 免疫层析; 多残留快速检测

Abstract: A rapid and sensitive colloidal gold immunochromatographic assay based on a monoclonal antibody,which could detect 11 fluoroquinolones(FQs) was developed. The monoclonal antibody was labeled with 20 nm gold particles as the detection reagent which was immobilized on glass fiber, and norfloxacin- ovalbumin was blotted on the test line while a sheep anti-mouse antibody was used on the control line combined on nitrocellose. The detection limits of the strip in pork and shrimp samples were 30 ng/g for ciprofloxacin, enrofloxacin, ofloxacin and 100 ng/g for norfloxacin, pefloxacin, enoxacin, marbofloxacin, lomefloxacin, danofloxacin, sarafloxacin and difloxacin. The whole process involved sample preparation and detection could be finished in less than 20 minutes. The results demonstrated that the method could be used as a screening tool for the determination of 11 FQs residues in large amount of pork and shrimp samples on site.

Key words: fluoroquinolones; colloidal gold; immunochromatography; rapid detection of multiple residues

中图分类号:

S859.79⁺9.9

冯婷婷, 刘一兵, 侯惠仁, 贾娟娟, 李振华, 韩世泉. 氟喹诺酮类药物胶体金试纸的研制[J]. 中国畜牧兽医.

FENG Ting-ting, LIU Yi-bing, HOU Hui-ren, JIA Juan-juan, LI Zhen-hua, HAN Shi-quan. Development of an Immunochromatographic Strip Test for Fluoroquinolones[J]. .

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