腹水综合征肉鸡空肠黏膜组织结构及相关基因表达分析

doi:10.16431/j.cnki.1671-7236.2019.06.031

Abstract

Abstract:

This experiment was aimed to study the relative expression of NF-κB and the tight junction protein(occludin,claudin-1 and ZO-1) mRNA and the changes of villus length (V),crypt depth (C) and V/C value in the development of broiler ascites syndrome,providing a theoretical basis for the prevention and treatment of broiler ascites syndrome.A total of 40 1-day-old Ross broilers were routinely reared for 7 days and randomly divided into control group and model group (added 3% lard and 4% fish meal to standard,added 0.12% NaCl to drinking water,and raising temperature at 9-11℃),and measured body weight at 35 days old.The ascites heart index was measured,and the jejunum tissue was taken for HE staining to measure the villus length and crypt depth.The gene expression of jejunal NF-κB and tight junction protein were detected by Real-time PCR.The results showed that compared with control group,the weight of broiler in model group was extremely significantly decreased (P<0.01),the ascites heart index was extremely significantly increased (P<0.01),the jejunum length and V/C value were extremely significantly decreased (P<0.01),the crypt depth was extremely significantly increased (P<0.01),the relative expression of NF-κB mRNA was extremely significantly increased (P<0.01),and the relative expression of tight junction proteins occludin,claudin-1 and ZO-1 mRNA were extremely significantly decreased (P<0.01).This results indicated that the intestinal mucosal structure and function abnormality and intestinal permeability increase promoted the development of broiler ascites syndrome.

Key words: broiler ascites syndrome; jejunum; intestinal villi; tight junction protein

CLC Number:

S856.5

WEN Shuang, GUO Yurong, YANG Lan, ZHAO Dongdong, WANG Zhuo, SHEN Weiqi, CHENG Jia, SUN Yaogui, WANG Wenkui, LI Hongquan, DUAN Zhibian. Analysis of Histological Structure and Expression of Related Genes in Jejunum Mucosa of Broilers Ascites Syndrome[J]. , 2019, 46(6): 1825-1831.

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Analysis of Histological Structure and Expression of Related Genes in Jejunum Mucosa of Broilers Ascites Syndrome

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