鸡HSP90AA1基因SNP检测及启动子区CpG岛的甲基化研究

doi:10.16431/j.cnki.1671-7236.2019.02.021

Abstract

Abstract:

This study was aimed to analyze the structure of HSP90AA1 gene,reveal the distribution of SNP in Wenchang chicken and Beijing-You chicken,investigate the promoter CpG islands methylation status of HSP90AA1 gene,and explore the role of HSP90AA1 gene in muscle tissue growth and development.HSP90AA1 gene was amplified by PCR.Then the gene mutation was identified by sequencing.CpG island was predicted using online software MethPrimer.With MassArray system,the DNA methylation levels and patterns of CpG islands in HSP90AA1 gene promoter were analyzed between the breast muscles of Wenchang chicken and Beijing-You chicken.Seven mutations were detected in the HSP90AA1 gene genomic fragment,two SNPs in the promoter region (A-189G,C-109T),one SNP in the first exon (A+6G),one SNP in the second exon (C+343T),two SNPs in the second intron (A+634G,A+836G) and one SNP in the seventh intron (A+3449G).The chicken HSP90AA1 gene sequence was determined including ten exons and nine introns.We found a CpG island,extending from -1 802 to -469 bp,in the promoter of HSP90AA1 gene.Forty-two CpG dinucleotides in HSP90AA1 promoter were tested.A total of 9 CpG loci (CpG_16.17.18,CpG_21.22.23,CpG_32.33 and CpG_57) were significantly different in different developmental stages in Wenchang chicken.A total of 4 CpG loci (CpG_1,CpG_5.6 and CpG_57) were significantly different in different developmental stages in Beijing-You chicken.This study proved that sequence information and methylation levels of HSP90AA1 gene in Wenchang chicken and Beijing-You chicken were different.The results provided some useful epigenetic basis principles for growth and development,system selection of Wenchang chicken and Beijing-You chicken.

Key words: Wenchang chicken; Beijing-You chicken; HSP90AA1 gene; promoter; methylation

CLC Number:

CHAO Zhe, WU Wangjun, XING Manping, HUANG Lili, SUN Ruiping, LIU Hailong, ZHENG Xinli. Detection of Single Nucleotide Polymorphism and Methylation of CpG Island in Promoter Region of Chicken HSP90AA1 Gene[J]. , 2019, 46(2): 504-511.

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Detection of Single Nucleotide Polymorphism and Methylation of CpG Island in Promoter Region of Chicken HSP90AA1 Gene

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