斑点叉尾鮰源致病性维氏气单胞菌的分离与生物学特性鉴定

doi:10.16431/j.cnki.1671-7236.2018.11.027

Abstract

Abstract:

In order to identify the agent of a serious disease occurred in Ictalurus punctatus,a dominant bacteria (GZTL2017) was isolated from diseased Ictalurus punctatus.It was identified by clinical anatomy observation,bacterial isolation and culture,gram stain microscopy,animal regression test,biochemical test,16S rDNA sequence analysis,partial virulence gene detection and drug susceptibility analysis.Clinical anatomical observations showed that the diseased Ictalurus punctatus showed surface ulcers,fin root bleeding,gills and symptoms of intestinal congestion.The isolate showed milky white colonies with moist surface,smooth and translucent edges,and uniform morphology in the medium.Gram stain microscopy showed that the isolate was single or double-existent,with straight-negative bacilli at both ends.Animal regression tests showed that the isolate had strong pathogenicity.Biochemical tests showed that the isolate was mobilized,with positive reactions such as oxidase,V-P and phenylalanine deaminase,and negative reactions such as arginine dihydrolase and H₂S.The 16S rDNA sequence of the isolate was more than 99% homology with that of Aeromonas veronii,and the isolate formed a cluster with other Aeromonas veronii reference strains by phylogenetic tree.Three virulence genes were detected from the isolate,including aerolysin (Aer),adhesion (Aha) and outer membrane protein A (OmpA) genes.The drug sensitivity test showed that the strain was sensitive to 14 drugs such as florfenicol,doxycycline and ofloxacin;It was moderately sensitive to 7 drugs such as norfloxacin,neomycin and vancomycin;It was resistant to 8 drugs such as medimycin,oxacillin and cefradine;And the minimal inhibitory concentrations of florfenicol and doxycycline were 1 and 2 μg/mL,respectively.This experiment successfully isolated a single strain of Aeromonas veronii,which provided a scientific basis for the prevention and treatment of Ictalurus punctatus infected with Aeromonas veronii.

Key words: Ictalurus punctatus; Aeromonas veronii; bacteria isolation; biological characterization

CLC Number:

S943

TIAN Lang, WEN Guilan, ZHANG Shengbo, YANG Baiqi, LI Changhong, XU Li, LIN Hanqing, GUAN Guodan. Isolation and Biological Characterization of the Pathogenic Aeromonas veronii from Ictalurus punctatus[J]. , 2018, 45(11): 3203-3210.

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Isolation and Biological Characterization of the Pathogenic Aeromonas veronii from Ictalurus punctatus

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