陇东肉牛PON1基因结构与功能的生物信息学分析

doi:10.16431/j.cnki.1671-7236.2018.07.003

Abstract

Abstract:

In order to clarify the structure and function of paraoxonase 1 (PON1) gene,all exon sequences of PON1 gene in Longdong cattle were amplified by PCR method,the complete sequence was assembled by Seqman software,and the base composition,open reading frame,physical and chemical properties of coding protein,atomic composition,number of amino acid residues,hydrophilicity and hydrophobicity,molecular weight,isoelectric point,secondary structure,higher structure,etc were predicted and analyzed by bioinformatics method.The results showed that the CDS sequence of PON1 gene in Longdong cattle was 1 068 bp,encoding 355 amino acids,Leu was the most abundant,Cys and Trp were the least.The content of A+T (56.55%) was higher than that of G+C (43.26%) in base composition.Compared with the published sequence (GenBank accession No.:EU289337) of cattle,we found that there were mutations which were a synonymous mutation in exons 4,6,8 and 9,but none of them changed amino acids.PON1 protein was a stable water-soluble protein,the atomic composition was C₁₈₁₀H₂₇₉₆N₄₅₄O₅₃₃S₁₂,the molecular mass was 39.83 ku,and the theoretical isoelectric point was 5.24.The number of amino acids of random coil,extended strand,α-helix and β-turn in the secondary structure of PON1 protein was 159,116,55 and 25,respectively,eventually formed a hybrid model with random coil and extended strand.Hydrophobic results of PON1 protein showed that the 8th Thr had the strongest hydrophobicity (the highest score:2.878) and the 299th Pro at the highest hydrophilicity (the lowest score:-2.222).The results of this study would lay a foundation for further study on the function of PON1 protein in Longdong cattle and explore the effect of PON1 gene mutation on the carcass and meat quality traits of local cattle in Gansu province.

Key words: Longdong cattle; PON1 gene; bioinformatics; mutation

CLC Number:

ZHANG Juan, MU Tong, ZHAO Ping, FENG Xiaofang, WU Jianping, GU Yaling. Bioinformatics Analysis of Structure and Function of PON1 Gene in Longdong Cattle[J]. , 2018, 45(7): 1750-1758.

References

[1] 孟冰,徐延勇,程力维,等.慢病毒载体表达外源PON1对小鼠巨噬细胞的影响[J].武汉大学学报(医学版),2015,36(1):1-5. MENG B,XU Y Y,CHENG L W,et al.Effects of exogenous PON1 expressed by letivirus vector on macrophages[J].Medical Journal of Wuhan University,2015,36(1):1-5.(in Chinese)
[2] MACKNESS M,MACKNESS B.Human paraoxonase-1(PON1):Gene structure and expression,promiscuous activities and multiple physiological roles[J].Gene,2015,567(1):12-21.
[3] 王帆,白涛,李鹏鹏,等.对氧磷酶的结构、活性及基因多态性与冠心病的关系[J].中国老年学杂志,2013,33:1203. WANG F,BAI T,LI P P,et al.Relationship between paraoxonase structure,activity,gene polymorphism with coronary heart disease[J].Chinese Journal of Gerontology,2013,33:1203.(in Chinese)
[4] DEAKIN S P,BIOLETTO S,BOCHATONPIALLAT M L,et al.HDL-associated paraoxonase-1 can redistribute to cell membranes and influence sensitivity to oxidative stress[J].Free Radical Biology & Medicine,2011,50(1):102-109.
[5] 习海玲,刘昌财,问县芳,等.磷酸三酯水解酶空间结构及催化水解机理研究进展[J].应用与环境生物学报,2015,21(3):392-400. XI H L,LIU C C,WEN X F,et al.Advances in research on spatial structure and catalytic mechanisms of phosphoric triester hydrolase[J].Chinese Journal of Applied & Environmental Biology,2015,21(3):392-400.(in Chinese)
[6] MIYOSHI M,NAKANO Y,SAKAGUCHI T,et al.Gene delivery of paraoxonase-1 inhibits neointimal hyperplasia after arterial balloon-injury in rabbits fed a high-fat diet[J].Hypertension Research,2007,30(1):85-91.
[7] KHERSONSKY O,TAWFIK D S.Structure-reactivity studies of serum paraoxonase PON1 suggest that its native activity is lactonase[J].Biochemistry,2005,44:6371-6382.
[8] PAUL H B.The inflammatory consequences of psychologic stress:Relationship to insulin resistance,obesity,atherosclerosis and diabetes mellitus,type Ⅱ[J].Medical Hypotheses,2006,67:879-891.
[9] CHEONG H S,YOON D,KIM L H,et al.Titin-cap (TCAP) polymorphisms associated with mar-bling score of beef[J].Meat Science,2007,77:257-263.
[10] RINCON J,MADEIRA E M,CAMPOS F T,et al.Exogenous paraoxonase-1 during oocyte maturation improves bovine embryo development in vitro[J].Reproduction in Domestic Animals,2016,51(5):827-830.
[11] 卫钦锋,李先加.血管内皮生长因子及对氧磷酶1基因多态性与糖尿病视网膜病变的相关性[J].重庆医学,2017,46(30):4223-4225. WEI Q F,LI X J.Correlation between vascular endothelial growth factor and paraoxonase 1 gene polymorphism with diabetic retinopathy[J].ChongqingMedicine,2017,46(30):4223-4225.(in Chinese)
[12] 郭朝群,孙建赟,袁莉莉,等.CYP2C19、PON1基因多态性与脑梗死患者氯吡格雷治疗预后的相关性研究[J].皖南医学院学报,2017,36(4):329-332. GUO C Q,SUN J Y,YUAN L L,et al.Correlation analysis between the CYP2C19 and PON1 gene polymorphism and prognosis of cerebral infraction patients following clopidogrel therapy[J].Acta AcademiaeMedicinae Wannan,2017,36(4):329-332.(in Chinese)
[13] 刘珂崎,于风旭,闫继强.PON1基因多态性与血小板再活化与支架内血栓的相关性研究[J].中国循证心血管医学杂志,2017,9(8):941-943. LIU K Q,YU F X,YAN J Q.Correlation among PON1 gene polymorphism,platelet reactivation and in-stent thrombosis[J].Chinese Journal of Evidence-Based Cardiovascular Medicine,2017,9(8):941-943.(in Chinese)
[14] 辛宏,李程亮,蔡天志,等.PON1基因多态性与PCI术后主要心血管事件相关性研究[J].陕西医学杂志,2017,46(4):417-418. XIN H,LI C L,CAI T Z,et al.Correlation between PON1 gene polymorphism and major cardiovascular events after PCI[J].Shaanxi Medical Journal,2017,46(4):417-418.(in Chinese)
[15] 李力,孙飞达,朱灿,等.陇东黄土高原区肉牛产业化及饲草资源生产潜力分析——以甘肃省平凉市崆峒区为例[J].中国畜牧杂志,2016,52(24):14-18. LI L,SUN F D,ZHU C,et al.Analysis on the industrialization of beef cattle and the production potential of forage grass in Longdong loess plateau——A case study of baobe district in Pingliang city,Gansu province[J].Chinese Journal of Animal Science,2016,52(24):14-18.(in Chinese)
[16] 徐飞,成述儒,罗玉柱.绵羊DRB1基因生物信息学分析[J].生物技术通报,2011,1:113-118. XU F,CHENG S R,LUO Y Z.Bioinformatics analysis of sheep DRB1 gene[J].Biotechnology Bulletin,2011,1:113-118.(in Chinese)
[17] TEIBER J F,DRAGANOV D I,LA DU B N.Lactonase and lactonizing activities of human serum paraoxonase (PON1) and rabbit serum PON3[J].Biochemical Pharmacology,2003,66(6):887-896.
[18] 赵晶晶.对氧磷酶1(PON1)基因多态性与2型糖尿病易感性的meta分析[D].石家庄:河北医科大学,2016. ZHAO J J.Association between paraoxonase 1(PON1) polymorphisms and type 2 diabetes mellitus susceptibility:A meta-analysis[D].Shijiazhuang:Hebei Medical University,2016.(in Chinese)
[19] CASTRO N A,PFEIFER L,ANDRADE J S,et al.Effect of serum paraoxonase-1(PON1) activity on follicular development and pregnancy rate in cattle[J].Animal Reproduction Science,2018,188:130-136.
[20] 姬爱国,周正奎,张路培,等.牛PON1基因对生长和胴体性状的影响[J].畜牧兽医学报,2009,40(1):122-128. JI A G,ZHOU Z K,ZHANG L P,et al.PON1 gene SNPs and association with growth and carcass traits in beef cattle[J].Acta Veterinaria et Zootechnica Sinica,2009,40(1):122-128.(in Chinese)
[21] 靳聪飞,梁婷玉,刘新峰,等.牛GUCY1A3和SFXN1基因的克隆及生物信息学分析[J].中国畜牧兽医,2017,44(2):357-364. JIN C F,LIANG T Y,LIU X F,et al.Cloning and bioinformatics analysis of GUCY1A3 and SFXN1 genes in cattle[J].China Animal Husbandry & Veterinary Medicine,2017,44(2):357-364.(in Chinese)
[22] 叶宇华,张倩倩,钟建美,等.人类珠蛋白相关基因上游开放阅读框的变异分析[J].遗传,2017,39(3):232-240. YE Y H,ZHANG Q Q,ZHONG J M,et al.Analysis of variants in upstream open reading frames of human globin-related genes[J].Hereditas,2017,39(3):232-240.(in Chinese)
[23] 李升,牛熙,徐霖,等.香猪对氧磷酯酶基因家族的克隆及序列分析[J].中国畜牧兽医,2012,39(7):15-19. LI S,NIU X,XU L,et al.Cloning and sequence analysis of paraoxonase gene family in Xiang pig[J].China Animal Husbandry & Veterinary Medicine,2012,39(7):15-19.(in Chinese)
[24] 姬爱国.牛8个基因的克隆、表达特征、原核表达、SNPs检测及其与部分性状的关联分析[D].杨凌:西北农林科技大学,2008. JI A G.Identification,characteration of expression,SNPs detection,prokaryotic expression and association analysis of the eight with part traits in beef cattle[D].Yangling:Northwest A & F University,2008.(in Chinese)

Bioinformatics Analysis of Structure and Function of PON1 Gene in Longdong Cattle

PDF (PC)

Knowledge

Abstract

Cite this article

share this article

References

Related Articles 15

Recommended Articles

Metrics

Comments

[1]	WANG Saiqiao, ZHAO Lu, ZHAI Zhenhan, ZHANG Binglei, JIA Wanhang, WANG Yuqin. Tissue Expression and Bioinformatics Analysis of miR-449a/b Precursor Sequences in Sheep [J]. China Animal Husbandry and Veterinary Medicine, 2023, 50(6): 2175-2184.
[2]	WEI Mingbang, BIANBA Qiongda, XIAO Qingqing, DUAN Mengqi, CHAMBA Yangzom, SHANG Peng. Cloning,Bioinformatics and Expression Analysis of CDKN1B Gene in Tibetan Pigs [J]. China Animal Husbandry and Veterinary Medicine, 2023, 50(6): 2196-2206.
[3]	LI Yongbin, YANG Huilin, SONG Zihao, ZHANG Yu, YANG Jin, MENG Jia, CUI Xiaozhen, LYU Xiaoling, ZHENG Mingxue, BAI Rui. Cloning and Bioinformatics Analysis of MIF Gene in Eimeria tenella [J]. China Animal Husbandry and Veterinary Medicine, 2023, 50(6): 2450-2459.
[4]	ZHANG Dianqi, MA Xinhao, YANG Zhimei, MEI Chugang, ZAN Linsen. Bioinformatics Analysis of PAI1 Gene and Its Effect on the Proliferation and Differentiation of Intramuscular Adipocytes in Qinchuan Beef Cattle [J]. China Animal Husbandry and Veterinary Medicine, 2023, 50(5): 1729-1741.
[5]	WANG Haorui, YU Baojun, YANG Jiayi, WEI Rong, FU Xi, WANG Chuanchuan, ZHANG Di, LI Desheng, CAI Zhengyun, GU Yaling, ZHANG Juan. Bioinformatics Analysis and Tissue Expression of DERA Gene in Jingyuan Chickens [J]. China Animal Husbandry and Veterinary Medicine, 2023, 50(5): 1764-1773.
[6]	CHEN Meiting, ZHENG Congsen, LIANG Zexian, LIN Qiaoer, CHANG Chuanzhe, ZHOU Jun, FENG Jun, LI Linlin, QIN Limei. Cloning and Bioinformatics Analysis of env Gene of Avian Leukosis Virus Subgroup J Strain [J]. China Animal Husbandry and Veterinary Medicine, 2023, 50(5): 1785-1795.
[7]	PAN Pengcheng, LEI Zongquan, LI Xian, HU Xiangyun, QIN Qiantao, QIN Zhaoxian, GUAN Zhihui, CHEN Baojian, XIE Bingkun. Bioinformatics Analysis,Eukaryotic Expression Vector Construction and Tissue Expression of MYL2 Gene in Luchuan Pigs [J]. China Animal Husbandry and Veterinary Medicine, 2023, 50(5): 1796-1806.
[8]	LIU Jialong, ZHANG Yiwen, ZHANG Yueran, SUN Qijuan, CHEN Jian, HE Lei, JIA Yanyan, DING Ke, YU Zuhua. Cloning,Prokaryotic Expression and Bioinformatics Analysis of ELAVL1 Gene in Chicken [J]. China Animal Husbandry and Veterinary Medicine, 2023, 50(5): 1807-1817.
[9]	ZHUO Na, ZHU Zhongwu, LI Jing, HU Shixiang, WANG Jianchang, LIN Hua, CHEN Chaolin, HAN Diangang, AI Jun, CHEN Peifu. Bioinformatics Analysis and Eukaryotic Expression of ORF2 Protein of Swine Hepatitis E Virus [J]. China Animal Husbandry and Veterinary Medicine, 2023, 50(5): 1959-1970.
[10]	TAO Qinghua, HUANG Caiyun, CHEN Chao, LIU Wei, SUN Ningning, LI Ang. Cloning and Tissue Expression Difference Analysis of LIF Gene in White Muscovy Duck [J]. China Animal Husbandry and Veterinary Medicine, 2023, 50(4): 1296-1306.
[11]	FAN Bingfeng, LI Wen, LIU Lixiang, ZHAO Xiangyuan, SHAO Jing, LIN Lefeng, SUN Huimin, ZHANG Xulin, XU Baozeng. Cloning and Bioinformatics Analysis of AANAT Gene in Mink [J]. China Animal Husbandry and Veterinary Medicine, 2023, 50(4): 1307-1318.
[12]	JIA Xinyue, MA Jing, ZHANG Yanyan, MA Xun, WANG Zhengrong, BO Xinwen. Bioinformatics Analysis and Prokaryotic Expression of EGR-07243 and EGR-07243 Genes of Echinococcus granulosus [J]. China Animal Husbandry and Veterinary Medicine, 2023, 50(3): 847-858.
[13]	LI Dan, ZHANG Haisen, WANG Yiqun, GAO Dengke, ZHAO Hongcong, LI Chao, JIN Yaping, CHEN Huatao. Eukaryotic Expression Vector Construction,Bioinformatics and Tissue Expression Profiles Analysis of CRY1 Gene in Dairy Cows [J]. China Animal Husbandry and Veterinary Medicine, 2023, 50(3): 870-881.
[14]	SONG Xingchao, MENG Jinzhu, ZHAO Yuanyuan, WU Zhenyang, AN Qingming. Cloning and Bioinformatics Analysis of MyoG Gene Promoter Region Sequence in Guizhou White Goat [J]. China Animal Husbandry and Veterinary Medicine, 2023, 50(3): 893-903.
[15]	ZHU Xihui, LI Yanlan, WANG Zhiwei, PANG Zheyu, LI Yubao, LIU Cheng, SI Zhenshu, LU Jianbiao, CAO Shengliang, XUE Xijuan. Whole Genome Sequencing of a Resistant Riemerella anatipestifer Strain and Analysis of Antibiotic Resistance and Genetic Evolution [J]. China Animal Husbandry and Veterinary Medicine, 2023, 50(3): 1140-1149.