O型口蹄疫病毒衣壳酸敏感性位点的筛选

doi:10.16431/j.cnki.1671-7236.2015.04.008

Abstract

Abstract: The study was aimed to select the acid sensitive sites of foot-and-mouth disease virus (FMDV) type O capsid. Using the bovine FMDV type O ON strain as the research object, six acid resistance monoclonal strains were obtained after treatment in pH 6.0acidic environment for serial passages. The P1 region which encoding their capsid proteins of the mutagenic viruses was sequenced and compared with the parental virus, then seven mutation sites were found, of which the mutation sites A1334G(Y445C), A1643G(Q548R), A1822G/A1823C (N608A) of P1 region found in six strains were sense mutation, while the mutation site G1371A was nonsense mutation. In addition the mutation site C1849T(P617S) of P1 region found in m2 was sense mutation and the mutation site G468T of P1 region found in m1 was nonsense mutation. This research laid a good foundation for the reveal of the acid sensitive mechanism and the acid sensitive enhance of FMDV type O capsid.

Key words: FMDV type O; capsid; acid sensitivity; acid resistance sites

CLC Number:

S855.3

XIE Yin-li, MA Qi, QIN Xiao-dong, YIN Xiang-ping, LIU Ji-xing, ZHANG Zhi-dong, LI Zhi-yong. Selection of the Acid Sensitive Sites of FMDV Type O Capsid[J]. , 2015, 42(4): 823-829.

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Selection of the Acid Sensitive Sites of FMDV Type O Capsid

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