China Animal Husbandry & Veterinary Medicine ›› 2014, Vol. 41 ›› Issue (9): 31-34.

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Research on the Production Process of Competitive ELISA Test Kit for Peste des Petits Ruminants Virus

LIU Xiao-hui1, YANG Yun-qing2, YE Ling-ling2, ZHU He2, LV Jian-qiang3, ZHAO Wen-hua4, YAN Hong1, YIN Shang-lian2, HUA Qun-yi3, YANG Shi-biao3, ZHANG Guang-pei2, ZHOU Xiao-li2, DONG Jun2, AI Jun2   

  1. 1. Baoding Entry-exit Inspection and Quarantine Burea, Baoding 071051, China;
    2. Yunnan Entry-exit Inspection and Quarantine Burea, Kunming 650228, China;
    3. Shenzhen Entry-exit Inspection and Quarantine Burea, Shenzhen 518045, China;
    4. Yunnan Animal Science and Veterinary Institute, Kunming 650224, China
  • Received:2014-03-24 Online:2014-09-20 Published:2014-09-24

Abstract: In order to research on the production process of competitive ELISA test kit for peste des petits ruminants virus (PPRV), in this test, nucleoprotein (N) protein expressed in insect cell and monoclonal antibody of PPRV were used to establish a high specificity, sensitivity and strong competitive ELISA method for detection of PPRV, and the competitive ELISA procedures were optimized. 977 sheep and cattle serum samples collected from Xizang and Inner Mongolia were assayed with this established competitive ELISA and rC-ELISA antibody kit for PPRV of the OIE at the same time. The detecting results displayed that specificity, sensitivity and accuracy were 99.89%,93.82% and 99.38%. The competitive ELISA method of PPRV established in this assay would provide the basis for research on the production process of ELISA test kit as well as a reference for prevention scientifically.

Key words: peste des petits ruminants virus (PPRV); nucleoprotein protein; monoclonal antibody; competitive ELISA

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