Abstract: In this experiment, the Brucella outer membrane protein 22 （omp22） gene was taken as the research object. Through the gene sequence cloning, expression vector construction, prokaryotic expression and affinity purification, expression and purification of the protein were studied. The results showed that the nucleotide sequence of omp22 gene contained 639 bp, which encoded 212 amino acids residues with predicted molecular weight of 22 ku. The electrophoresis results showed that molecular weight of recombinant omp22 protein was 47 ku, which was consistent with the theoretical value. All these results provided a good basis for immunity stimulation and immunity protective effect of recombinant omp22 protein in further research.

Key words:

Brucella; outer membrane protein 22 gene; gene cloning; prokaryotic expression; purification